We have our grossing staff cover all GI biopsies, breast biopsies and endometrial curettings or any small colorless tissue with 0.1% basic fuchsin. It leaves it a nice pinkish tint after processing, the specimens are visible in the paraffin, and we haven't had any pathologist complaints.
On Fri, Oct 22, 2010 at 8:10 AM, <dkb...@chs.net> wrote: > As much as I respect Dr. Richmond, I would have to disagree that staining > bx's with eosin is a waste of pathologist time. It helps the embedding > tech and cutting tech see the minute pieces, which may be otherwise lost. > Sometimes that is the diagnostic material. > We would not want to put a patient through another procedure because we > couldn't recover the tissue submitted. > We use a vial with a dropper. Once the biopsy is placed in the cassette > you take 1 second more to drop a drop of eosin on the specimen. > Well worth everyone's time in my humble opinion. > > > Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical > Center I > 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: > 804-765-5582 l dkb...@chs.net > > > > > > > > Robert Richmond <rsrichm...@gmail.com> > Sent by: histonet-boun...@lists.utsouthwestern.edu > 10/22/2010 10:44 AM > > To > histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] Re: Eosin to dye small Biopsies > > > > > > > Allison Scott at LBJ Hospital in Houston, Texas asks about the use of > eosin to dye small biopsy specimens. > > Several replies mention addition of eosin to one of the processing > alcohols. I have never seen this done, in maybe 60 pathology services > I've worked in. (I'd know, because I nearly always examine the > paraffin block when I order recuts or send a case out for > consultation.) > > It's a fine time-waster for the pathologist to mark small specimens > with dye while grossing. I've used Mercurochrome (merbromin, related > to eosin but with 26% mercury) which fortunately was banned in the USA > about ten years ago. I've used eosin, and I've used safranin (from the > microbiology lab's Gram stain setup). I don't know whether safranin > interferes with FISH, as eosin is well known to, nor do I know if you > can put safranin in the processing alcohol. And I've used Davidson > tissue marking inks. > > I've never seen or heard of cobalt blue used for this purpose - is > this the insoluble coloring material, chemically cobalt aluminate? > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet