We don't, Leica recommends that you don't. We have our grossing group squirt a 0.1% Basic Fuchsin on the small translucent or white specimens. It leaves it light pink like the eosin.
On Fri, Oct 22, 2010 at 11:09 AM, Rathborne, Toni < trathbo...@somerset-healthcare.com> wrote: > Does anyone use eosin in their Peloris? > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Shea's > Sent: Friday, October 22, 2010 2:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] eosin in processor > > > This is the best kept secret....For those who have never tried this, you > don't know what you are missing in the ease of embedding and cutting. > > We have been adding alcoholic eosin (about 10cc each) to the 1st & 2nd 100% > alcohols, leaving the last 100% pure (of course there is carry over during > the week), then we dump the first and rotate the other 2 each week. The one > that we now moved into position #2 will get 10cc eosin). > > We send our IHC & FISH to a HUGE reference lab. I specifically asked about > this interfering and the technical rep said that it is not a problem esp > they see a lot of it and they know to avoid non specific perimeter staining > (the Pathologists know how to read around this because many labs are using > Eosin in the processors). > > J > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet