Hi
We do a lot of IHC on mouse lymph nodes, unfixed, fresh-frozen in OCT.
Sometimes individual lymph nodes will peel away from the OCT as they are
being cut, and then curl up, making them impossible to get a good
section. In the same mold several will be fine and one will curl up.
Cutting further on the same block, the problem lymph node may be fine.
Any ideas on what variables determine this, either in the initial
freezing, or in the cutting? Would it help to soak the tissue at room
temp in the OCT for a period of time before freezing? Dip in PBS before
freezing?
Thanks for any insights.
Ed
--
Edward Roy, PhD
Professor of Pathology
Professor of Molecular and Integrative Physiology
University of Illinois at Urbana-Champaign
506 S. Mathews Ave.
Urbana, IL 61801
217 333-3375
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