I used to do a "stain" on post mortem heart slices to look for recent infarctions. the entire tissue turned a sort of bluey colour which remained through formain fixation and processing. the tissue however was incubated in solution of NBT in a sodium cyanide containing buffer - not sure if that would still be allowed these days - but if you want - i could send you the protocol we used
best regards On Thu, Oct 28, 2010 at 8:51 PM, Laurie Colbert < laurie.colb...@huntingtonhospital.com> wrote: > I previously posted a question regarding Nitroblue Tetrazolium Chloride, > but I didn't really receive the info that I needed - so I thought I > would ask again. > > > > I need to purchase this item for a research doc. He wants to immerse > tissue in this solution for 24 hours and then process as usual. It is > my understanding that this is some kind of dye. I see that I can order > it from Sigma in tablet form. I've seen it from other companies in a > powder form. > > > > Has anyone ever used this reagent in the capacity that I describe? Is > it available as a ready-to-use solution/liquid? Is there a certain > strength/percentage that I should use? > > > > Thanks, > > Laurie Colbert > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet