Hi all, after more than a decade of NOT cutting frozen sections, I find myself back at the ice-face. To get my hand in (not literally) I thought I would do some trial sections on stored tissue - stuff that was in formalin and now in 70% alcohol.
Horror ; dismay. The tissue, once frozen, is all mushy in the middle. Is this because of teh long storage? is there anything I can do to improve the situation? much appreciated -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet