I would just add that you do not need more than 2 hours total time in paraffin before embedding.
Date: Wed, 10 Nov 2010 10:16:43 -0500 From: Merced M Leiker <lei...@buffalo.edu> Subject: Re: [Histonet] Paraffin Tissue Crumbles To: Michael Mashore <mmash...@vapop.ucsd.edu>, histonet@lists.utsouthwestern.edu Message-ID: <449016b996b2d4cdc661e...@cdywxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=utf-8; format=flowed Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each. Regards, Merced --On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore <mmash...@vapop.ucsd.edu> wrote: > Hello Histonet Users, > > > > I have just started using paraffin and am having many difficulties. Most > of the time my tissue crumbles when sectioning. I have no real experience > in paraffin histology and have been given the task of becoming proficient > by myself, so I am hoping for feedback as to why my tissue keeps > crumbling. The tissue in question has been: skeletal muscle, cardiac > muscle, liver, and brain (all from rat). > > > > The tissue was fixed in 10% neutral buffered formalin for 7 days at 4°C > and then transferred to an automated tissue processor, with the following > schedule: > > > > 2 hours 70% dehydration alcohol > > 2 hours 80% dehydration alcohol > > 2 hours 95% dehydration alcohol > > 2.5 hours 95% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > .5 hour Hemo-De > > .5 hour Hemo-De > > .5 hour Hemo-De > > 1 hour paraffin > > 4 hours paraffin > > > > They were infiltrated for 1 hour without vacuum then embedded. > > The blocks were stored in the freezer before cutting. > > The knife angle was 5°. > > Sections were 5µm thick. > > > > I would appreciate any feedback whatsoever. John Ronan Research Associate Bioanalytics & Pathology 732 594-6378 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, November 10, 2010 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 84, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: looking for references to stain protocols (Roberta Horner) 2. Jaclyn Bhamornsiri - Important Message (jaclyn_bhamorns...@vwr.com) 3. Histology Lab Survey (Lesley Bechtold) 4. Cutting and embedding (Lin Bustamante) 5. NSH Validation WS attendees (Patsy Ruegg) 6. how to fix floating sections? (Caroline Bass) 7. Part-time Lab opportunity in Scottsdale, AZ (Carlos Rodriguez, MD) 8. Paraffin Tissue Crumbles (Michael Mashore) 9. Histo data needed (O'Donnell, Bill) 10. RE: Distance Learning (joelle weaver) 11. Oil Red O trivia (Finlay Finlay) 12. Re: Oil Red O trivia (Geoff McAuliffe) 13. Oil Red O control (Inman, Anna) 14. Sending Slides Out in St. Louis, MO (Alyssa Peterson) 15. Re: Oil Red O control (Drew Meyer) 16. RE: Oil Red O control (Sun,Yuping) 17. Re: Paraffin Tissue Crumbles (Merced M Leiker) 18. RE: Oil Red O control (Weems, Joyce) 19. RE: Paraffin Tissue Crumbles (Nails, Felton) 20. RE: Sending Slides Out in St. Louis, MO (Langenberg, Stacey) 21. Position - Muscle Enzyme Histochem Tech (Barone, Carol ) 22. NH Histology Job Opportunity (Alisha Dynan) 23. New York Dermpath Lab Manager (Alisha Dynan) 24. Laboratory Manager position in MI (Alisha Dynan) 25. Histology Supervisor Job in Oklahoma (Alisha Dynan) 26. Histology Supervisor Position in GA (Alisha Dynan) ---------------------------------------------------------------------- Message: 1 Date: Tue, 9 Nov 2010 13:44:52 -0500 From: Roberta Horner <r...@psu.edu> Subject: [Histonet] RE: looking for references to stain protocols To: "Burton, Lynn" <lynn.bur...@illinois.gov>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <f12bb68c2ba80b4686a8ea261f80dd459907399...@pdc.padlspsu.psu.edu> Content-Type: text/plain; charset="us-ascii" This is the reference that I have for this stain. Andersen A.A. & VanRompay D (2005). Chlamydiosis. In: A Laboratory Manual for the Isolation and Identification of Avian Pathogens, Fifth Edition. Submitted USA. Roberta Horner HT/HTL Animal Diagnostic La Penn State University -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Tuesday, November 09, 2010 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] looking for references to stain protocols I am in need of references for stain protocols. The stain I am searching for in particular is the modified Gimenez, also known as Pierce-Vanderkamp for chlamydia and rickettsia. If anyone can point me in the right direction I would appreciate it. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 9 Nov 2010 14:00:43 -0500 From: jaclyn_bhamorns...@vwr.com Subject: [Histonet] Jaclyn Bhamornsiri - Important Message To: histonet@lists.utsouthwestern.edu Message-ID: <ofd207faae.dae0822e-on852577d6.00686f8d-852577d6.00686...@vwr.com> Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 11/09/2010 and will not return until 12/31/2014. As of Nov 9th, 2010, I am no longer with VWR. If you need assistance, pls contact VWR Customer Service at 1800-932-5000 (or vwrcustomerserv...@vwr.com). For more urgent matters, pls contact Leize Lessig at 770-733-8190 (or leize_les...@vwr.com). Thanks! Jackie B. ------------------------------ Message: 3 Date: Tue, 9 Nov 2010 14:14:48 -0500 From: Lesley Bechtold <lesley.becht...@jax.org> Subject: [Histonet] Histology Lab Survey To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <3bda51ffd1a83b4e90829f594a5c371743316ee...@jaxbhmail01.jax.org> Content-Type: text/plain; charset="us-ascii" Dear Histonetters, Scientific Services at The Jackson Laboratory is conducting benchmarking surveys in an effort to develop an understanding of the current best practices in operations, management and technical delivery at peer institutions. The goal is to gather quality data that can be used to assist us and all survey participants with operational assessment and planning. Survey participants will receive compiled results which will be de-identified before distribution. If you would like to participate, just click on the link below and answer the questions in the survey. Additionally, for those of you who do EM, there is a link to an EM Facility survey as well. It will only take a few minutes of your time. With thanks in advance! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) Histo Survey - http://www.surveymonkey.com/s/5RPSYG2 EM Survey - http://www.surveymonkey.com/s/5RGYH9W ------------------------------ Message: 4 Date: Tue, 09 Nov 2010 12:34:59 -0600 From: "Lin Bustamante" <lbustama...@cvm.tamu.edu> Subject: [Histonet] Cutting and embedding To: <histonet@lists.utsouthwestern.edu> Message-ID: <4cd93ff3020000b9000e5...@cvm.tamu.edu> Content-Type: text/plain; charset=US-ASCII Could you please tell me what is the average for embedding surgical blocks in one hour? Is there an average for cutting? Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas A&M University College Station, TX 77843-4458 ------------------------------ Message: 5 Date: Tue, 9 Nov 2010 13:29:37 -0700 From: "Patsy Ruegg" <pru...@ihctech.net> Subject: [Histonet] NSH Validation WS attendees To: "'ihcrg Group \(E-mail\)'" <ih...@googlegroups.com>, <histonet@lists.utsouthwestern.edu>, "'National Society of Histotechnology'" <hi...@nsh.org> Cc: "'Morken, Tim'" <timothy.mor...@ucsfmedctr.org>, diane.tokug...@kp.org Message-ID: <78b71368e2844d3e9b0e2253896f8...@patsyoffice> Content-Type: text/plain; charset="us-ascii" For those of you who attended the IHC ws at NSH on Validation by Tim Morken and Patsy Ruegg we have posted the rest of the information we promised you to my ftp://ihctech.net <ftp://ihctech.net/> site for you to download. We provided the user name and pass word you will need in the workshop. The folder is labeled "Tim Morken" and you can download the files to your computer. Remember this site is for uploading and downloading not opening. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email <mailto:pru...@ihctech.net> pru...@ihctech.net web site <http://www.ihctech.net> www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ Message: 6 Date: Tue, 9 Nov 2010 21:51:02 +0000 From: Caroline Bass <cb...@wfubmc.edu> Subject: [Histonet] how to fix floating sections? To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c8ff2c75.1344%cb...@wfubmc.edu> Content-Type: text/plain; charset="Windows-1252" Hello Everyone, I have a bunch of rat brains that I have frozen in isopentane and stored at -80. I'd like to collect 300 micron sections on a cryostat and collect tissue punches for RNA isolation. I would then like to take the remaining tissue and stain. There are a few ways of doing this, what would work the best is to take two 50 micron section, followed by a 300 micron, followed by two more 50, and go through the brain serially. I will then stain the 50 micron sections with nissl or thionin, so that the structures are easily identified and then use these to direct the tissue punches. The second 50 micron sections would be reserved for GFP IHC. Is this feasible? Here are some direct questions: 1. how would I store the 300 micron sections? 2. Any suggestions/protocols for staining the 50 micron sections for structure? Which stain would you use? 3. Can I post-fix the 50 micron sections? How about the 300 micron? One reason I want to use floating sections for the nissl/thionin is that I can mount these very flat, no wrinkles and I'm very comfortable working with floating sections. I don't want to risk precious tissue trying to optimize this. One methodology recommended to me is to freeze a formalin solution in a 24 well plate, put the section on top flat and let them thaw together at room temperature or 4 degrees. This gives the tissue a nice, slow fixation. Any other suggestions? How about working with the RNA? I'm thinking of using RNAlater-ice for example. Does anyone know if this screws up native fluorescence of GFP? Thanks! Caroline Bass ------------------------------ Message: 7 Date: Tue, 9 Nov 2010 15:47:21 -0700 From: "Carlos Rodriguez, MD" <cfr...@gmail.com> Subject: [Histonet] Part-time Lab opportunity in Scottsdale, AZ To: histonet@lists.utsouthwestern.edu Message-ID: <aanlktikat=rr7tl1qzdkdcft_-pf9in-wg1j=w8nz...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi all. We have a part-time job opportunity for someone interested primarily in accessioning and grossing skin specimens at our in-house dermatopathology lab (North Scottsdale Dermatology). A small amount of general lab management will also be required (ie, maintaining CLIA logs, ordering supplies etc). The hours needed will likely be Monday, Wednesday and Thursday mornings (~5 or 6am-9am). The start date will be around late December. The hours and start date are flexible, and the workload is very reasonable. Qualifications are either HT certification, or at a minimum an Associate's Degree in a health-related field with college-level credit in Chemistry, Biology, and Math, in addition to work experience in grossing and accessioning skin specimens. If interested, please respond to me by email and include a CV and/or description of your qualifications. Thanks! Carlos Rodriguez, MD ------------------------------ Message: 8 Date: Tue, 9 Nov 2010 14:56:49 -0800 From: "Michael Mashore" <mmash...@vapop.ucsd.edu> Subject: [Histonet] Paraffin Tissue Crumbles To: <histonet@lists.utsouthwestern.edu> Message-ID: <652586e049884d8491a0e70448595...@vandeberg> Content-Type: text/plain; charset="iso-8859-1" Hello Histonet Users, I have just started using paraffin and am having many difficulties. Most of the time my tissue crumbles when sectioning. I have no real experience in paraffin histology and have been given the task of becoming proficient by myself, so I am hoping for feedback as to why my tissue keeps crumbling. The tissue in question has been: skeletal muscle, cardiac muscle, liver, and brain (all from rat). The tissue was fixed in 10% neutral buffered formalin for 7 days at 4°C and then transferred to an automated tissue processor, with the following schedule: 2 hours 70% dehydration alcohol 2 hours 80% dehydration alcohol 2 hours 95% dehydration alcohol 2.5 hours 95% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol .5 hour Hemo-De .5 hour Hemo-De .5 hour Hemo-De 1 hour paraffin 4 hours paraffin They were infiltrated for 1 hour without vacuum then embedded. The blocks were stored in the freezer before cutting. The knife angle was 5°. Sections were 5µm thick. I would appreciate any feedback whatsoever. Thank you very much. Michael ------------------------------ Message: 9 Date: Tue, 9 Nov 2010 16:39:27 -0700 From: "O'Donnell, Bill" <billodonn...@catholichealth.net> Subject: [Histonet] Histo data needed To: histonet@lists.utsouthwestern.edu Message-ID: <e5bf2d5bfdcdfd49882f9df0b9f1a609a1f...@chimsx016.chi.catholichealth.net> Content-Type: text/plain; charset=us-ascii Those who work in similar labs please help me out: Could anyone tell me how a clinical histology lab that do 6500 cases a year is staffed. (Average aboout 70 blocks a day, 20-30% small biopsies that require 3 levels so about 120-140 "ribbons" a day) We do not do cyto processing, we have automated H&E.and coverslipping. All special stains and immuno is does manually, but volume is low, 2-4 SS a week and maybe 6 antibodies. We do gross probalbly 60-70% of cases and assist at gross for the rest. We have no aides and are staffed @ 1.5 FTE. I'm trying to justify some help, but the suits need numbers to crunch. I hope that is enough info for comparison. Thanks in advance, Bill 'the tired tech" O'Donnell Kearrney, NE ------------------------------ Message: 10 Date: Wed, 10 Nov 2010 10:47:57 +0000 From: joelle weaver <joellewea...@hotmail.com> Subject: RE: [Histonet] Distance Learning To: <dch...@yahoo.com>, Histonet <histonet@lists.utsouthwestern.edu>, <jweav...@cscc.edu> Message-ID: <snt135-w62044634e6908b279bfa94d8...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Hi Phyllis We offer an on line, distance learning program from Columbus State Community Colllege that is fully accredited by NAACLS. It awards a certificate in histotechnology and meets the training and eligibility requirements for the ASCP HT registry examination. The certificate program is linked to an Associates degree in the Department of Allied and Multi-competency Health for those wanting a degree option. We are currently accepting students for the next program year. Our application and admissions process and application form is available from our program web page at www.cscc.edu/histology We are looking to expand our market and meet the needs of students and laboratories outside of Ohio. We have a few clinical affiliates in the Midwest, other than Ohio, and we would like to extend this further into under-served areas, ( as far as histology schools). We are able to offer 24/7 access to materials, instruction in theory and practice, practical work assessment, and interactive delivery via Blackboard learning platform. In the future we plan to offer continuing education opportunities that are histology specific to those aready certified or who may participate in the ASCP- CMP program ,and want to meet the continuing education requirements needed for participation. If I may answer any questions about histology training, certification or the on line study of histology, please feel free to contact me at jweav...@cscc.edu or at 614-287-2217. Joelle Weaver HTL (ASCP) > Date: Mon, 8 Nov 2010 07:26:59 -0800 > From: dch...@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Distance Learning > > We are thinking of using one of the online or distance learning programs to > help > in the training of new histotechs. Has anyone had any experience with any of > these programs, what colleges offer the program, pros, cons. Any feedback is > welcome. > > Thanks > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 10 Nov 2010 14:24:32 -0000 From: "Finlay Finlay" <f.fin...@formed.gla.ac.uk> Subject: [Histonet] Oil Red O trivia To: <histonet@lists.utsouthwestern.edu> Message-ID: <ded6e938ef692144990ebefadeaebb62894...@formed-ex.formed.internal> Content-Type: text/plain; charset="us-ascii" Hello Just wondering if anyone knows the what the 'O' in oil red O stands for. I suspect that it stands for nothing other than O similar to the 'G' in Orange G. One of our pathologists is looking for a tricky stain question to annoy trainees with. Thanks for your help Finlay ------------------------------ Message: 12 Date: Wed, 10 Nov 2010 09:39:10 -0500 From: Geoff McAuliffe <mcaul...@umdnj.edu> Subject: Re: [Histonet] Oil Red O trivia To: Finlay Finlay <f.fin...@formed.gla.ac.uk> Cc: histonet@lists.utsouthwestern.edu Message-ID: <4cdaae8e.20...@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed So, the pathologist does not know the answer but he wants to annoy trainees? Glad I don't work for him! Geoff Finlay Finlay wrote: > Hello > > Just wondering if anyone knows the what the 'O' in oil red O stands for. > I suspect that it stands for nothing other than O similar to the 'G' in > Orange G. One of our pathologists is looking for a tricky stain question > to annoy trainees with. > > Thanks for your help > > Finlay > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ********************************************** ------------------------------ Message: 13 Date: Wed, 10 Nov 2010 07:45:28 -0700 From: "Inman, Anna" <anna.in...@stmarygj.org> Subject: [Histonet] Oil Red O control To: "Geoff McAuliffe" <mcaul...@umdnj.edu>, "Finlay Finlay" <f.fin...@formed.gla.ac.uk> Cc: histonet@lists.utsouthwestern.edu Message-ID: <2925ae271eaad440af48fcceb8002d091443e...@smgmail01.smgj.sclhs.net> Content-Type: text/plain; charset="us-ascii" Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 14 Date: Wed, 10 Nov 2010 09:51:19 -0500 From: Alyssa Peterson <aly...@alliedsearchpartners.com> Subject: [Histonet] Sending Slides Out in St. Louis, MO To: histonet@lists.utsouthwestern.edu Message-ID: <aanlktinyjncfvsgqtm0o0rnf+e4rfg7lc7o3aa6_t...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hello, A friend of mine who has a brand new laboratory in St. Louis, MO currently has their histotech prepare their slides but are looking for a laboratory to have them read at because they do not have a Dermatopathologist. Please contact me if you have lab in the area who would be interested in doing that for them. Thank you! -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ------------------------------ Message: 15 Date: Wed, 10 Nov 2010 09:54:46 -0500 From: Drew Meyer <41dm...@gmail.com> Subject: Re: [Histonet] Oil Red O control To: "Inman, Anna" <anna.in...@stmarygj.org> Cc: histonet@lists.utsouthwestern.edu Message-ID: <aanlktimkncbrb-ix-62azg2ejdpotquo88yftjhee...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna <anna.in...@stmarygj.org> wrote: > Hello - > > How does everyone handle having a positive control for Oil Red O - We > very infrequently have this stain and have had difficulty keeping > control on hand. > > > > > > Thank you > > Anna > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 16 Date: Wed, 10 Nov 2010 10:11:41 -0500 From: "Sun,Yuping" <s...@pathology.ufl.edu> Subject: RE: [Histonet] Oil Red O control To: "'Drew Meyer'" <41dm...@gmail.com>, "'Inman, Anna'" <anna.in...@stmarygj.org> Cc: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <530a57d46c74f34b862f95e3f4dff3228d5d2...@hsc-cms01.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" Hello Histonet Users, I have a question about Oil Red O staining. Could the Oil Red O solution(Poly Scientific, Cat.No. S1848) be reused? I would appreciate any feedback whatsoever. Thank you very much. Cathy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Wednesday, November 10, 2010 9:55 AM To: Inman, Anna Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil Red O control There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna <anna.in...@stmarygj.org> wrote: > Hello - > > How does everyone handle having a positive control for Oil Red O - We > very infrequently have this stain and have had difficulty keeping > control on hand. > > > > > > Thank you > > Anna > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 10 Nov 2010 10:16:43 -0500 From: Merced M Leiker <lei...@buffalo.edu> Subject: Re: [Histonet] Paraffin Tissue Crumbles To: Michael Mashore <mmash...@vapop.ucsd.edu>, histonet@lists.utsouthwestern.edu Message-ID: <449016b996b2d4cdc661e...@cdywxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=utf-8; format=flowed Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each. Regards, Merced --On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore <mmash...@vapop.ucsd.edu> wrote: > Hello Histonet Users, > > > > I have just started using paraffin and am having many difficulties. Most > of the time my tissue crumbles when sectioning. I have no real experience > in paraffin histology and have been given the task of becoming proficient > by myself, so I am hoping for feedback as to why my tissue keeps > crumbling. The tissue in question has been: skeletal muscle, cardiac > muscle, liver, and brain (all from rat). > > > > The tissue was fixed in 10% neutral buffered formalin for 7 days at 4°C > and then transferred to an automated tissue processor, with the following > schedule: > > > > 2 hours 70% dehydration alcohol > > 2 hours 80% dehydration alcohol > > 2 hours 95% dehydration alcohol > > 2.5 hours 95% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > .5 hour Hemo-De > > .5 hour Hemo-De > > .5 hour Hemo-De > > 1 hour paraffin > > 4 hours paraffin > > > > They were infiltrated for 1 hour without vacuum then embedded. > > The blocks were stored in the freezer before cutting. > > The knife angle was 5°. > > Sections were 5µm thick. > > > > I would appreciate any feedback whatsoever. > > > > Thank you very much. > > > > Michael > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ Message: 18 Date: Wed, 10 Nov 2010 10:20:27 -0500 From: "Weems, Joyce" <jwe...@sjha.org> Subject: RE: [Histonet] Oil Red O control To: "Inman, Anna" <anna.in...@stmarygj.org>, Geoff McAuliffe <mcaul...@umdnj.edu>, Finlay Finlay <f.fin...@formed.gla.ac.uk> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e164044a071...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Smear mayonnaise on a slide.. It works well. And of course - not the fat free kind! j -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Inman, Anna Sent: Wednesday, November 10, 2010 09:45 To: Geoff McAuliffe; Finlay Finlay Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O control Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 19 Date: Wed, 10 Nov 2010 09:25:47 -0600 From: "Nails, Felton" <flna...@texaschildrens.org> Subject: RE: [Histonet] Paraffin Tissue Crumbles To: "'Merced M Leiker'" <lei...@buffalo.edu>, "Michael Mashore" <mmash...@vapop.ucsd.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c1fe5960057c084ca389ce97779062908c70b...@tcdmsg01.ad.texaschildrenshospital.org> Content-Type: text/plain; charset=iso-8859-1 I agree most of your times are too long, but you can still get sections if you put water on your ice block and allow the tissue to rehydrate a bit or put a little ammonium hydroxide and water on your ice and sit your blocks to be section in this solution. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Wednesday, November 10, 2010 9:17 AM To: Michael Mashore; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Tissue Crumbles Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each. Regards, Merced --On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore <mmash...@vapop.ucsd.edu> wrote: > Hello Histonet Users, > > > > I have just started using paraffin and am having many difficulties. > Most of the time my tissue crumbles when sectioning. I have no real > experience in paraffin histology and have been given the task of > becoming proficient by myself, so I am hoping for feedback as to why > my tissue keeps crumbling. The tissue in question has been: skeletal > muscle, cardiac muscle, liver, and brain (all from rat). > > > > The tissue was fixed in 10% neutral buffered formalin for 7 days at > 4°C and then transferred to an automated tissue processor, with the > following > schedule: > > > > 2 hours 70% dehydration alcohol > > 2 hours 80% dehydration alcohol > > 2 hours 95% dehydration alcohol > > 2.5 hours 95% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > 2 hours 100% dehydration alcohol > > .5 hour Hemo-De > > .5 hour Hemo-De > > .5 hour Hemo-De > > 1 hour paraffin > > 4 hours paraffin > > > > They were infiltrated for 1 hour without vacuum then embedded. > > The blocks were stored in the freezer before cutting. > > The knife angle was 5°. > > Sections were 5µm thick. > > > > I would appreciate any feedback whatsoever. > > > > Thank you very much. > > > > Michael > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================================== ------------------------------ Message: 20 Date: Wed, 10 Nov 2010 08:30:22 -0700 From: "Langenberg, Stacey" <stacey.langenb...@ucdenver.edu> Subject: RE: [Histonet] Sending Slides Out in St. Louis, MO To: Alyssa Peterson <aly...@alliedsearchpartners.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1f70fcbb6d4ec549b2adf69b9f9eac0348f696a...@steamboat.ucdenver.pvt> Content-Type: text/plain; charset="us-ascii" Hi Alyssa, We are not in St Louis but I work for a really great Dermpath here in Colorado who might read them for you. "People are not an interruption of our business. People are our business." Stacey Langenberg HT (ASCP) QIHC Laboratory Manager Histology/IF CU Dermatopathology Consultants 1999 N. Fitzsimons Pkwy Suite 120 Aurora, CO 80045 Lab-720-859-3559 Fax- 303-344-0789 Office- 303-577-2303 Cell-970-405-7742 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson [aly...@alliedsearchpartners.com] Sent: Wednesday, November 10, 2010 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sending Slides Out in St. Louis, MO Hello, A friend of mine who has a brand new laboratory in St. Louis, MO currently has their histotech prepare their slides but are looking for a laboratory to have them read at because they do not have a Dermatopathologist. Please contact me if you have lab in the area who would be interested in doing that for them. Thank you! -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 10 Nov 2010 11:00:57 -0500 From: "Barone, Carol " <cbar...@nemours.org> Subject: [Histonet] Position - Muscle Enzyme Histochem Tech To: <histonet@lists.utsouthwestern.edu> Message-ID: <37e4bac017f57141af64faa5aeb04ce8033a8...@wlmmsx01.nemours.org> Content-Type: text/plain; charset="us-ascii" Hello Histonetters: We presently have a position available for a HT/HTL (certified, or eligible) in our clinical muscle enzyme histochemistry lab (within the Department of Biomedical Research). We request 3 yrs experience minimum in EHC... and skills for manual IF, as well. If you meet these requirements and have interest, please contact cbar...@nemours.org. We are looking for you! ------------------------------ Message: 22 Date: 10 Nov 2010 11:55:24 -0500 From: Alisha Dynan <ali...@ka-recruiting.com> Subject: [Histonet] NH Histology Job Opportunity To: histonet@lists.utsouthwestern.edu Message-ID: <607630384.1289408124244.javamail.cfserv...@sl4app4> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am currently working on a Histology Supervisor and Histology Technologist job opportunity in Southern NH. These job opportunities are with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salray and benefits. If interested in learning more, please email me your resume to ali...@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 23 Date: 10 Nov 2010 11:57:53 -0500 From: Alisha Dynan <ali...@ka-recruiting.com> Subject: [Histonet] New York Dermpath Lab Manager To: histonet@lists.utsouthwestern.edu Message-ID: <777306831.1289408273737.javamail.cfserv...@sl4app4> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am currently working on a Laboratory Manager position in NY. the ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in dermatopathology. This job opportunity is with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to ali...@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 24 Date: 10 Nov 2010 11:58:16 -0500 From: Alisha Dynan <ali...@ka-recruiting.com> Subject: [Histonet] Laboratory Manager position in MI To: histonet@lists.utsouthwestern.edu Message-ID: <1327844936.1289408296253.javamail.cfserv...@sl4app1> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am currently working on a Laboratory Manager position in MIY. the ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in dermatopathology. This job opportunity is with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to ali...@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 25 Date: 10 Nov 2010 11:59:26 -0500 From: Alisha Dynan <ali...@ka-recruiting.com> Subject: [Histonet] Histology Supervisor Job in Oklahoma To: histonet@lists.utsouthwestern.edu Message-ID: <768330664.1289408366500.javamail.cfserv...@sl4app1> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am currently working on a Histology Supervisor position in OK. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to ali...@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 26 Date: 10 Nov 2010 12:00:45 -0500 From: Alisha Dynan <ali...@ka-recruiting.com> Subject: [Histonet] Histology Supervisor Position in GA To: histonet@lists.utsouthwestern.edu Message-ID: <2144143006.1289408445189.javamail.cfserv...@sl4app2> Content-Type: text/plain; charset="utf-8" Hi Histonet Members, I am currently working on a Histology Supervisor position in GA. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. The candidate must also be interested/willing to live in a rural area but work in a large teaching hospital (600+ beds). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to ali...@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 84, Issue 11 **************************************** Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet