Wow this sounds like a molecular biology question that will have to be figured out and probably no one has done that yet for this particular situation. Please write it up if you do figure it out.
Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ejsch...@ucalgary.ca Sent: Monday, November 15, 2010 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] glutaraldehyde fixed tissues and genomic DNA Hello, I have PFA+glutaraldehyde fixed tissues that I might want to digest down in order to extract genomic DNA suitable for bisulfite sequencing. The tissues are embryonic mouse heads. The fixative is a PFA + glutaraldehyde mix: 3.6 PFA with 5% glut. The glut is grade 1 or electron miccroscopy grade. I'd like to pro-K the tissues and then phenol-chloroform extract genomic DNA. My hope is that the amount and quality of the DNA will be suitable for bisulfite conversions for methylation anaylsis. Will the DNA be abundant? Will it be fairly protein free (does the fixative fix protein to the DNA?). Thanks Eric University of Calgary Facutly of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet