I am cutting whole embryos and neonates. I would prefer to avoid paraffin ( I seem to have issues with varying consistency of tissue between different organs. Ie some are very friable while others cut perfectly all in the same block) and cut frozen on a sliding microtome. However I need to keep the pieces from separating and keep them in the proper orientation. I was going to use gelatin but the pieces fall out even when I vacuum embed and fix the gelatin. I have just joined the list so I don't know all the passed posts. I did see some about HistoGel. Would this allow me to embed with histogel and cut frozen on a sliding microtome and retain a "complete section " that I could then mount without losing pieces?
Also If I use Histogel first would this improve the issues I have with paraffin. Lastly has anyone cut paraffin sections on a sliding microtome. How do you set it up? Blade angle , Perpendicular to block or angled? Thanks Alex _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet