We've always only used hematoxylin where ever I've worked. I haven't embedded in eons so can't tell you how visible those specks of tissue are.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, December 01, 2010 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Small biopsy specimens We have been using eosin to stain our small biopsies prior to tissue processing so that they are easier to see when embedding. Recently one of our PA's started using Hematoxylin instead of the eosin, because that is what they used where she trained. Upon further investigation the manufacturers of both of our tissue processors discourage the use of eosin because it is very hard on the working parts of the machines and requires more PM's to prevent damage. So we are considering switching to using Heme instead. Do any of you out there use Heme on your biopsy specimens? Do your embedders find it easier to see these tiny tissues when embedding? Any pros or cons greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet