Yea I would say make sure the samples are getting fixed long enough, fixation is also temp dependent so if it is colder now you might have to fix longer, try and fix your tissue, even biopsies for 24 hours if you can in 10% Neutral phosphate buffered formalin.
Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Monday, December 20, 2010 2:30 PM To: 'wassan alkadhumi'; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] need ur help Check the fixative the biopsies are going into. Is it 10% neutral buffered formalin? Are they being fixed for long enough? Remember these are usually small, thin biopsies so they do not need extended processing so use the time to extend the fixation. You could try placing the biopsies in fixative in a warm oven (37-45oC) before loading onto the processor. Are they being allowed to dry before being placed in fixative? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wassan alkadhumi Sent: Tuesday, 21 December 2010 4:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need ur help Hi all Hope u r all doing well, am an Iraqi histotech working in both histopathology and IHC department in Iraq. One part of what i do is IHC (manual) i have no problem with, the other part is what i have problems with, Renals and Livers, we do regular H &E stain , PAS and Masson Tirchrome and all of them were text book quality until ten days ago, the problem is the Stains even the H&E are not Fine, its smugish. And the most important stain for the histopathologist is the Trichrome which it should be fine and delacate and its not. I checked the procedures and stain preparations and i doing it the correctly , I made new solutions and did prolonged deparaffinization 10 min each step (3 xylenes, 2 100%ETOH, one 90%ETOH, one 70%ETOH) and there was no difference. I should mention that those problems happened with only Renal and Liver biopsies the H&E and PAS on other types of tissues are working just great. the one thing that changed before 10 days ago is the wether it became really cold and our lab temp. is not controlled. Am going crazy please help me Thank u a lot Wassan Histotech. Shorsh hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet