It may however if you're doing immunofluorescence, as MB has been used
to quench autofluorescence (Seeing the Wood through the Trees: A Review
of Techniques for Distinguishing Green Fluorescent Protein from
Endogenous Autofluorescence; Nicholas Billinton and Andrew W. Knight,
Analytical Biochemistry 291, 175–197 (2001). It isn't likely to alter
antigen-antibody binding if that's what you meant.
Mike King
UF Pharmacology & Therapeutics
Message: 1
Date: Sat, 1 Jan 2011 15:37:32 -0700
From: "Patsy Ruegg" <pru...@ihctech.net>
Subject: RE: [Histonet] methylene blue stained tissue
To: "'Quinn, Tim L.'" <quin...@umkc.edu>,
<histonet@lists.utsouthwestern.edu>
Cc: "'Herndon, Betty L.'" <hernd...@umkc.edu>
Message-ID: <47914cbc2e9b4deb80235f70df65b...@prueggihctechlt>
Content-Type: text/plain; charset="us-ascii"
Should not be a problem if you are using hrp/dab brown or ap/red detection.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Quinn, Tim
L.
Sent: Thursday, December 30, 2010 12:30 PM
To: histonet@lists.utsouthwestern.edu
Cc: Herndon, Betty L.
Subject: [Histonet] methylene blue stained tissue
Hello Histies,
I received rodent dermis that has been stained with methylene blue.
I would like to perform immunolabeling experiments on this tissue.
Should I expect any interference from the methylene blue stain?
Tim Quinn
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