Hello, I have a problem with what appears to be flecks of paraffin coming off of my slides in my first change of alcohol in my H&E stain. My pathologist indicates seeing "drops of unstained areas on the tissue", and I suspect it is from unsatisfactory deparaffinization. I use clear-rite 3 xylene subst. and have always done 3 changes at 3 min each before going to 100% alcohol. I have contacted the company and they claim that nothing is wrong with the product. I have tried changing out all stations to fresh clear rite 3 and increasing the time to 5 min each. I have also increased the temp on my slide oven (65 for 20min). All to no avail. Could this not be the problem? Could it be the paraffin? I use paraplast plus.
I would love to hear ANY suggestions other than what I have already tried! I'm starting to really worry now since the problem has persisted for a few months. Thank you! Patty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet