Hello Histonetters, While trying to eliminate any variables - processing times, embedding paraffin, waterbath temperature, time and temp in oven, deparaffinization, antigen retrieval, antibody manufacturer and lot # - I still seem to have a slight inconsistency with 1 of my immuno stains. Mast Cell Tryptase staining for colon biopsies. Can anyone offer any suggestions or ideas as to why this might be occuring? Even within my current control slides I see a nice distinct brown or a slightly more pale stain. I would love to hear your ideas. Thank you! Ann.BennettAnn
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