We process bone marrow biopsies the same day received. We use a combination decal/ fixative solution (Cal Rite decal) with good results. We keep in this solution a minimum of 3 hours before moving to tissue processor where first 2 solutions are a total of 3 hours in formalin. Although most bone marrow biopsies are received by 2:00 - 3:00pm; we've had some received as late as 4:00pm with good results. Our clinicians place the bone marrow core in pre-filled formalin at the point of collection, also.
The Cal Rite is a combination of formaldehyde, formic acid and methanol. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry Sent: Friday, January 14, 2011 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcifying bone marrows after processing I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification. She asked if we could process them and then decalcify and I have yet to find an answer to this question. Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
