I cc'd (is that a word?) the histonet for you. If you ever want to pose a question to everyone email: histonet@lists.utsouthwestern.edu.
PPE: Of course everyone has different levels of PPE to be worn, but I think the standard rule is... Gloves Lab Coat Mask (especially if cutting frozen lung tissue, it could have TB) Eye Wear You have to remember you need all of this because once the tissue hits room temperature it is basically fresh unfixed tissue again. I have cut frozen sections with no mask before and it was all ok, but I always wore one with lung frozens. Good Luck, hope this helped Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: Setlak, Lisa [mailto:lset...@childrensmemorial.org] Sent: Wednesday, January 19, 2011 10:08 AM To: Sarah Goebel Subject: RE: Caspase Hi Sarah, I don't have an answer to your question but was wondering if you could post one for me- I've tried multiple times and it doesn't seem to go through. Would you be able to tell me how to pose a question to the whole group or could you possibly post one "What type of PPE is normally worn when performing frozen sections"? Thanks in advance for your help. Lisa Van Valkenberg Histology Mgr. Children's Memorial Hospital Chg,. IL -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Wednesday, January 19, 2011 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Caspase Look...I am a plethora of questions today!! Has anyone ever used the antibody Caspase? I hear lymph node is a good positive control, but where have people purchased the antibody, and at what dilution for FFPE tissue? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet