We have been having the same problem recently. We have tried extending the washes after the hematoxylin, agitation and adding an additional wash. Nothing has helped. It is not every slide as you say, but random ones. We are using Gill 3 and eosin from Stat Lab. We have changed the stainer and processor a number of times since this began.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Mike Pence Sent: Wednesday, January 19, 2011 1:10 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E Stain More often than I would like to admit when I have seen this type of problem it has been that there is a solution out of place on the processor or the stainer. I would start there. Mike -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, January 19, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Stain Hello to all in histoland and Happy New Year. We are having issues with our H&E stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more.
_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet