We've done them for years...
6 levels on 2 slides - L1-L3, L4-L6 1st level placed near label end of
the slide No unstained.
We do C4d when ordered.
For cases with no previous biopsies - Pick up unstained slides at level 3 for:
PAS, Masson Trichrome, Iron, Congo Red and
Sulfonated Alcian Blue
Best, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
jeffery.mil...@spectrum-health.org
Sent: Thursday, January 20, 2011 12:06
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Myocardial Biopsies
My hospital has recently started performing heart transplants and we are now
receiving numerous myocardial biopsies to check for rejection. One of our
forensic pathologists is an expert in cardiovascular pathology and has read
many biopsies over the years for other hospitals. His protocol for cutting
these biopsies is very labor intensive (10 slides with 8 serial sections on
each slide, some stained with H&E others kept unstained and 2 for C4d immunos).
I'm curious to know what other labs are doing for these biopsies in hopes of
getting this protocol changes.
Thanks for your help
Jeff Miller
Spectrum Health
Grand Rapids. MI
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