If the tissue is not fixed soon after death the microscopic morphology
will be terrible, no matter whether the animal is refrigerated or
frozen. The person suggesting refrigeration/freezing is (obviously) not
familiar with histological technique.
As someone else on the list suggested, better planning is needed for the
harvesting of tissues for microscopy.
Geoff
On 1/21/2011 5:05 AM, krishna_adhik...@mail.com wrote:
Dear Group Members,
I have a strange query, One person suggested, if the animals for necropsy are
more and one can not handle the load of necropsy on the same day.
Then simply after sacrificing the animals refrigerate the dead animals to
reduce the autolysis process and then proceed with the actual necropsy
procedures.
is it possible to to the necropsy procedures on the freezed animals samples
for further histopathology evaluation?
expert comments are suggested.
Regards
krishna
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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