Hello,
We have to measure the "g ratio" (ratio of the inner axonal diameter to the 
total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per 
nerve will be evaluated with a standard imaging soflware.
Do you think that we can perform this work with thin paraffin slides - 1.5µm 
(stained with toluidine blue), in spite of the best practice seems to prepare 
semithin sections from epoxy resin blocks.
Thank you,
Michel

Michel BATAILLON
Histology
Evreux, France

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