Hi All, Does anyone know a company that sells cutting patches? The ones we use are black with an adhesive back that "sticks" to our cutting board. Seems like the company we were getting them from no longer carries them..
Thanks for your help Myra Huth,HT(ASCP) Bay Medical Center Pathology Dept. Panama City, Florida -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, January 25, 2011 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 86, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. re: storing acetic acid (Smith, Allen) 2. Re: Banana smelling chemical in our formalin (Robert Richmond) 3. Re: gram stain (Robert Richmond) 4. RE: g ratio (Houston, Ronald) ---------------------------------------------------------------------- Message: 1 Date: Tue, 25 Jan 2011 16:34:52 +0000 From: "Smith, Allen" <asm...@mail.barry.edu> Subject: [Histonet] re: storing acetic acid To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <aee9d3b1ba593a49b23da5a6a375654c188a2...@ex2010-02.barrynet.barry.edu> Content-Type: text/plain; charset="us-ascii" We have separate storage cupboards for inorganic acids (many of which are oxidizing agents) and organic acids (many of which are flammable). If we didn't have an organic acids cupboard, we would store it with organic solvents. The reaction of acetic acid with nitric acid might be violent, the reaction of acetic acid with ethanol is slow and quiet. > -Allen A. Smith > Professor of Anatomy > Barry University School of Podiatric Medicine ------------------------------ Message: 2 Date: Tue, 25 Jan 2011 11:31:49 -0500 From: Robert Richmond <rsrichm...@gmail.com> Subject: [Histonet] Re: Banana smelling chemical in our formalin To: histonet@lists.utsouthwestern.edu Message-ID: <AANLkTi=sk+zhj-k8-cickozrdd3cybokslzj3musz...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Amyl acetate ("banana oil") is produced by an alternate fermentation pathway by some of the yeasts used to make Hefeweizen type beers. The thought of recycling such a fine beer horrifies me. Ethyl acetate, formed by esterification of ethanol with acetic acid as fixatives such as Davidson's and O-Fix age, smells like airplane dope (if you're old enough to remember airplane dope) or nail polish remover (if you aren't). Obviously these fixatives could easily be mixed with formalin for recycling, since they also contain formaldehyde. About 25 years ago there was a fad for "odor masked" formaldehyde solutions. The ones I used smelled even worse than formaldehyde, but they didn't smell like banana oil. I don't know if these solutions are still available. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Tue, 25 Jan 2011 11:49:57 -0500 From: Robert Richmond <rsrichm...@gmail.com> Subject: [Histonet] Re: gram stain To: histonet@lists.utsouthwestern.edu Message-ID: <aanlktin2tiuhuyx8v2omi_hwa4fd5lqtiozbj7rff...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 The original Brown-Hopps tissue gram stain was published in Lee Luna's AFIP Manual, 3rd ed. 1968. It did not require picric acid, ethyl ether, or acetone. I remember that the stain was widely used after that. The AFIP manual method does require ethylene glycol monoethyl ether (Cellosolve 1), which is less of a fire hazard than is ethyl ether. See the MSDS at http://www.jtbaker.com/msds/englishhtml/e2600.htm before ordering it, though. Freida Carson (in the 2nd edition of her book - I don't have the 3rd) restored the picric acid and acetone in her modification of the Brown-Hopps method. Putting on my pathologist hat now - tissue gram stains are greatly overrated - they don't work nearly as well as they do on smears. If you want to see or count bacteria in tissue sections, use a simple blue stain (toluidine blue, or Diff-Quik II or its generic equivalent). Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 4 Date: Tue, 25 Jan 2011 12:13:16 -0500 From: "Houston, Ronald" <ronald.hous...@nationwidechildrens.org> Subject: [Histonet] RE: g ratio To: 'Bataillon Michel' <michel.batail...@citox.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e02e1309b208f94c83b968e45781001a234c53b...@nchexmbx01.columbuschildrens.net> Content-Type: text/plain; charset="iso-8859-1" Michel, Most studies involving morphometric studies of peripheral nerves involve "thick" plastic sections stained with toluidine blue. In my opinion, you are unlikely to get consistent and acceptable staining results on paraffin sections for the simple reason that the intense blue staining on thick EM sections is more from the osmium tetroxide than the toluidine blue per se. Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org<mailto:ronald.hous...@nationwidechildrens.org> www.NationwideChildrens.org<http://www.NationwideChildrens.org> -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bataillon Michel Sent: Tuesday, January 25, 2011 5:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] g ratio Hello, We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging soflware. Do you think that we can perform this work with thin paraffin slides - 1.5µm (stained with toluidine blue), in spite of the best practice seems to prepare semithin sections from epoxy resin blocks. Thank you, Michel Michel BATAILLON Histology Evreux, France ************************************************************* Ce message et toutes les pièces jointes sont confidentiels et établis à l'attention exclusive de ses destinataires. Si vous recevez ce message par erreur, merci de le détruire et d'en avertir immédiatement l'expéditeur. Toute utilisation de ce message non conforme à sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite. Internet ne permettant pas de garantir l'intégrité de ce message, le CIT décline toute responsabilité au titre de ce message s'il a été altéré, déformé ou falsifié. Protégeons ensemble l'environnement, n'imprimons ce mail (et ses pièces jointes) que si cela est nécessaire. 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