Hello everyone,
I am working on a protocol for a dual fluorescent stain with two mouse antibodies (one is IgG1 and the other is IgG2a). We started by performing the two stains sequentially with a serum free protein blocking step in between, tagging one antibody with a 488 FITC labeled secondary and a 568 Cy3 secondary on the other (both goat anti mouse of course). With this method we are getting a lot of background staining so I was wondering if anyone who has experience in this sort of work if you have a recommendation of a proper blocking solution between the sequential stains? I've heard about using Rodent Block Rat from Biocare Medical or an un-conjugated goat anti mouse IgG secondary immunoglobulin but I would like to hear from you all as well! :-) Thanks, Ross _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet