I think it is a very small minority of markers that would be sensitive to the methanol residues present in formalin. So it depends on what you plan on staining. For general purposes, I would always go with formalin, unless there is a proven need for formaldehyde.
__________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Québec, Canada J7H 1N8 tel: 450-435-9482 ext.247 fax: 450-435-4795 jm.lapoi...@accellab.com ------------------------------ Message: 3 Date: Fri, 28 Jan 2011 13:15:19 -0500 From: Jan Berry <jebe...@umich.edu> Subject: [Histonet] fixation question for IHC To: histonet@lists.utsouthwestern.edu Message-ID: <1ba205bb-22b9-4167-a36d-01b7f3f04...@umich.edu> Content-Type: text/plain; charset=us-ascii Is there a difference between using paraformaldehyde and neutral buffered formalin when choosing a fixative for IHC? I would prefer to use formalin because of easier preparation, but am willing to put in the extra time to make fresh paraformaldehyde solution if there is a compelling reason. Jan Berry University of Michigan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
