Hi, We are called to support an asthmatic rat lung project and need to stain for mast cells, eosinophils, neutrophils and possibly other inflammatory cells, such as Th2 cells etc. Any suggestions you can give us in terms of fixatives and special stains or IHC are very much appreciated. From my search, it seems that Carnoy's fixative with toluidine blue is the way to go for mast cells. But can one clearly differentiate eos from neuts with this fixative or a formalin fixed H&E stained lung section? If we need to do Trichrome, PAS, May Grunwald Giemsa and H&E etc. what would be the best fixative to accommodate these stains?
BTW, I heard that formalin free Zn-fixative is good for IHC. Will it be good for all these stains above? Sorry for so many questions. It shows how little experience I have in this area. Thank you, Shu Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
