I believe the two most recent articles from ASCO/CAP on this subject are Hammond, M.E., D.F. Hayes, M. Dowsett, D.C. Allred, K.L. Hagerty, S. Badve, P.L. Fitzgibbons, G. Francis, et al. 2010. American Society of Clinical Oncology/College Of American Pathologists guideline recommendations for immunohistochemical testing of estrogen and progesterone receptors in breast cancer. J Clin Oncol 28:2784-2795.
Wolff, A.C., M.E. Hammond, J.N. Schwartz, K.L. Hagerty, D.C. Allred, R.J. Cote, M. Dowsett, P.L. Fitzgibbons, et al. 2007. American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. J Clin Oncol 25:118-145. If you do not have access to the literature you can request reprints from the authors or it may be available on the ASCO website? Luis -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Liz Chlipala Sent: Wednesday, February 02, 2011 2:28 PM To: [email protected]; [email protected] Subject: RE: [Histonet] Her2 Fixation Requirement Lori I would think both if you could since the Her2 paper does address those issues as potentials for problems in testing variation (time to fixation). I believe the CAP/ASCO paper states not less then 6 and no more than 48 hours for fixation (2007 paper). I believe you also should document type of fixative if possible; vendor, lot number and expiration date if you can. I have pdfs of these documents if you need them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Wednesday, February 02, 2011 12:16 PM To: [email protected] Subject: RE: [Histonet] Her2 Fixation Requirement What is the appropriate time it should be in formalin? Do you start your time when it is initially put into formalin, or the start from the time it was dissected? Lori A Disher Fawcett Memorial Hospital Port Charlotte, FL 33952 [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
