Hello everyone, I am trying to stain for cartilage in mouse skull (cross-sectioning with the brain in tact) and wanted to know if anyone might have a good protocol for Safranin O / Fast green or Safranin O / von Kossa? The tissue is embedded in MMA and was cut on a saw microtome and finished down to an approximate 20 to 30 micron tissue thickness - 50 microns including the glue layer (I glue a slide to the block before making the cut and then grind and polish to finish). I tried Weigert's hematoxylin / Safranin O / Fast Green ( I began with Weigert's Hematoxylin, followed by Fast Green and finished with Safranin O), but the Safranin O seemed to overpower the Fast Green (the calcified bone stained more red than green). Any help on this issue would be greatly appreciated as always. Thanks again.
Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
