Hi Paula, You mentioned the staining in a liver specimen using the cell marque antibody against TTF-1. The clone named 8G7G3/1 that cell marque sells is known to stain liver cells and liver cancer. Your pathologist might be interested in knowing that. Here is a reference you can give him: http://www.nordiqc.org/Run-23-B5/Assessment/Assessment-TTF-1.htm It also lists another clone that doesn't cross react with liver.
Mark On Thu, Feb 3, 2011 at 12:38 PM, Paula Lucas <plu...@biopath.org> wrote: > How can I eliminate the background or as you say, non-specific staining? > > > > I'm no expert here, I admit, and so I'm asking for your help and > suggestions. I have searched the Histonet archives, and a lot of what I'm > seeing deals with animal or rodent tissues, and a lot of it was confusing > to > me. > > > > To give you a little background info: > > We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell > Marque. We were having issues with this marker from Lab Vision, so we > switched to TTF-1 a while ago. We use the UltraVision LP detection kit > from > Lab Vision. It's a polymer driven detection kit. > > > > The antibody is a ready to use, and we have the time set at 30 minutes. > > > > We were getting the background staining on a liver specimen last week, and > we also had this problem today on a lung case. My doctor is getting > frustrated, and wants me to do something about this, and to repeat the > TTF-1 > stain on the lung, so if someone can give me some suggestions to try, I'm > ready to try them. > > > > Thanks in advance, > > Paula > > Lab Manager > > Bio-Path Medical Group > > Fountain Valley, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet