Yes, we've used both the AP Red and DAB Refine kits manually with comparable results to the automation
Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarka Lhotak Sent: Friday, February 04, 2011 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bond Refine Red for manual IHC Hello all, I wonder if anyone is using the Bond Polymer Refine Red Detection kit (cat # DS390, Leica) for manual IHC. I've seen some beautiful staining, the technical specialist at Leica, however, told me it is only for Leica automatic stainers. It is an AP stain. Has anybody tried it? Thanks a lot, Sarka Lhotak McMaster University Hamilton, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet