Nancy, We get areas that appear brown when the tape doesn't lay flat or adhere to the slide. Most of the time this happens on decal slides or hard tissue where the tissue may be lifted a little. Try recoverslipping. Also, if it's not happening on these types of tissue, you may need more xylene dispensed. Sometimes, we just have to resort to the old-fashioned way of coverslipping with mounting media. I've never used the Klinipath tape. I prefer to stick with Sakura's brand - don't want to take any chances!
Laurie Colbert -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Sent: Friday, February 04, 2011 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Artifact or dirt on slides using a tape cover slipper? At our facility with have 2 Tissue-Tek automated tape cover slippers. The brand of tape that we use is made by a company called Klinipath, KP Tape (dist. By Mercedes Medical). On occasion we get complaints that the slides appear to have areas of dirt or dust on them. It appears to be on the inside. Has anyone else that uses a tape cover slipper ran across this particular problem? If so, what did you do to troubleshoot? If it is the tape, is there another brand or type that is preferable? Nancy Mitchell Pathology Arts, Inc Director of Sales and Marketing 951-270-0605 909-732-1666-Cell na...@pathologyarts.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet