Greetings Histoland,
I need some help. We are about to switch IHC machines from the Richard-Allen
Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run
for the validation study? We have over 100 primary antibodies. Normally, when
we work up a new antibody, we start with a titer. Once that is established, we
run 10 cases to check for specificity. Hopefully we can obtain cases that are
really positive, some weakly positive and some flat out negative. Once that is
completed, we run 10 different tissue types to check for any unexpected
cross-reactivity.
The ultra holds 30 slides and we are receiving two machines. If we run 10
slides/antibody, that's going to take a while, not to mention the number of
detection kits that will be used. Do you think 5 slides/antibody is sufficient?
I emailed CAP last week for their take and they never returned my email (I told
my medical director to hold their check for the year and see how fast they
respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are
your thoughts? Thanks
Joe (JTT)
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