Have you checked the temperature of the heater used to dry you slides? We ran into this problem a couple of years ago and the temp of the oven was too high.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeline Gi Sent: Friday, February 11, 2011 1:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E stain uneven Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why. Can someone tell me how does a routine H&E stain unevenly? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated…. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you
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