Room Temperature for 10-15 minutes and wash well with DI H2O after. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, February 11, 2011 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saliva for glycogen hydrolysis Those who use human saliva rather than diastase - for how long do you apply the saliva? At what temperature? Do you cover the saliva and section with a coverslip during treatment? I used to do this many years ago, but have been using diastase for so long now that I don't remember the particulars of the saliva method, and few books seem to provide that information. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
