Does it also work on human? Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pru...@ihctech.net web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Jan Shivers [mailto:shive...@umn.edu] Sent: Thursday, February 17, 2011 9:39 AM To: Patsy Ruegg; 'Amy Lee'; 'histonet' Subject: Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section Abcam has a rabbit anti-neutrophil elastase that works on rat FFPE tissues, according to their website. Jan Shivers ----- Original Message ----- From: "Patsy Ruegg" <pru...@ihctech.net> To: "'Amy Lee'" <amylee...@yahoo.com>; "'histonet'" <histonet@lists.utsouthwestern.edu> Sent: Thursday, February 17, 2011 8:49 AM Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section I do not know of a neutrophil specific ab for human ffpe tissue. You could do just DAB without blocking with h202 for myleoperoxidase but it will not distinguish between eosinophils and neutrophils myeloperoxidase will stain all myeloid cells with granules in the cytoplasm (granulocytes). Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Wednesday, February 16, 2011 1:07 PM To: histonet Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section Hello, I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section. Could anybody recommend a good antibody? Thanks in advance, Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet