Amy, We have used Abcam rabbit anti human myeloperoxidase (Ab9535) with very good results on FFPE rat tissue. No retrieval required, primary at 1:50, biotinylated secondary and ABC elite with DAB for detection. Hope that helps.
Jason Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jason.pal...@svhm.org.au Message: 7 Date: Wed, 16 Feb 2011 12:06:41 -0800 (PST) From: Amy Lee <amylee...@yahoo.com> Subject: [Histonet] neutrophil antibody on rat FFP section To: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <544851.36245...@web38005.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello, I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section. ? Could anybody recommend a good antibody? ? Thanks in advance, ? Amy Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet