I assume you mean what reagent to use as the negative control? The ideal control is a mixture of negative mouse serum and negative rabbit specifically titered to match the antibody concentration in the cocktail, which is probably proprietary. The next best solution is to use a universal negative control serum. The universal component typically denotes use with rabbit and mouse antibodies.
Will Chappell, HTL(ASCP)QIHC Histologist at large Sent from my iPhone On Feb 21, 2011, at 8:07 AM, Carol Bryant <cb...@lexclin.com> wrote: > I would like that information also. > Thank you, > Carol > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton > Sent: Sunday, February 20, 2011 3:00 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Pin4cocktail negative controls > > Hello Histonetters, > > What are you folks using as a negative control when running Pin4 cocktail > antibody. > > Thanks in advance, > MG > </PRE> > <html> > <body> > ------------------------------------------------------------<br /> > This email message, including any attachments, is for the sole use of the > intended recipient(s) and may contain information that is proprietary, > confidential, and exempt from disclosure under applicable law. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > have received this email in error please notify the sender by return email > and delete the original message. Please note, the recipient should check this > email and any attachments for the presence of viruses. The organization > accepts no liability for any damage caused by any virus transmitted by this > email.<br /> > ================================= > </body> > </html> > <PRE> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > NOTICE OF CONFIDENTIALITY > > This message, including any attachments, is intended only for the sole use of > the addressee and may contain confidential or privileged information that is > protected by the State of Kentucky and/or Federal regulations. If you are > not the intended recipient, do not read, copy, retain or disseminate this > message or any attachment. If you have received this message in error, please > call the sender immediately at (859)258-4000 and delete all copies of this > message and any attachment. Any unauthorized review, use, disclosure, copying > or distribution is strictly prohibited. Neither the transmission of this > message or any attachment, nor any error in transmission or misdelivery shall > constitute waiver of any applicable legal privilege. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
