My problem in understanding your difficulty is the definition: what is "poor nuclear artifact"? No detail? Weak staining? Strong staining? "Empty" nuclus? The cause of the problem will vary depending on its definition. Unless you are more specific I think it will be very difficult in trying to help you. Please be more specific René J.
--- On Fri, 2/25/11, Marshall, Kimberly K <kkmarsh...@anthc.org> wrote: From: Marshall, Kimberly K <kkmarsh...@anthc.org> Subject: [Histonet] Poor nuclear artifact To: histonet@lists.utsouthwestern.edu Date: Friday, February 25, 2011, 3:34 PM Happy Friday Histo people!!! I need some opinions on a processing issue I cant seem to fix. I run a short processing run each night for my small biopsies. With two processors, I rotate so the machine with the newest reagents is the one we run as the biopsy machine. For several weeks now my Pathologist have had issues with one or two blocks a day or one day it was one piece of tissue of many in a GI biopsy block,(only one piece of like 5 were affected the rest were fine) that they are saying has "poor nuclear artifact" . Most days it is only one block and has happend only once with tissue from the long run. This happens no matter a weekend or over night, fresh reagents or not. I have biopsies come from all over Alaska and in talking with them as well as checking the containers they are submitting in 10% NBF, The stainer is rotated daily so its not a Xylene not clearing the slide problem. I know sections can be thicker in a ribbon but the Pathologist dont seem to think that is the problem either. I have run out of things it could be and could really use some advise. Thanks and have a great weekend _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet