Hi Martha, I haven't used it, but you can find some info on it from the human protein atlas here:
http://www.proteinatlas.org/ENSG00000176887/antibody They recommend 1:100 and you can check out Sigma's recommended procedure for using their Prestige antibodies here: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/ 1/ihc_procedure.Par.0001.File.tmp/ihc_procedure.pdf Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Thursday, March 03, 2011 1:54 PM To: Histonet Subject: [Histonet] SOX11 antibody Hello all, I have been asked to try to work up the SOX11 antibody for a research project. The antibody they gave me is from Sigma Life Science. I am having difficulty getting any staining at all. If anyone has any suggestions I would be very grateful. We are using a Leica Bond 3. Thanks in advance for any help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet