Hello, We have been storing our slides in small zip loc bags at -20 and have data suggesting that slides stored in this fashion are reasonably well preserved after 3- 5 years. This was done with TMA slides. Was not done for special stain slides but for IHC.
Helen -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pru...@ihctech.net Sent: Tuesday, March 15, 2011 8:56 AM To: Harrison,Sandra C. Cc: histonet@lists.utsouthwestern.edu; Amador, Amanda Subject: RE: [Histonet] Control Slides I do not melt dry my cut control slides until i need to use them,=d i do store them in boxes at 4c, i have recently run into this problem =th blocks, so now i seal all my blocks with paraffin and store them in th�ridge as well. Patsy -------- Original Message -------- Subject: RE:=istonet] Control Slides From: "Harrison, Sandra C." <[1]sandra.harris...@va.gov> Date: Mon,=rch 14, 2011 1:40 pm To: "Lee & Peggy Wenk" <[2]lpw...@sbcglobal.net>, "Amador, Amanda" &l=[3]aama...@ameripath.com>, &=;[4]histo...@lists.utso uthwestern.edu> We dry the control slide at the same time tha=e dry the tissue being stained, since controls are supposed to be han=ed in the same manner as the test tissue. That being said, I guess if = were truly following that rule, we would cut the control on the same �y we cut the test sample. :-) "Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissu= so that the air doesn't touch the tissue during the months before=e slide is used in a stain." -----Original Message----- From: [5]histone=boun...@lists.utsouthwestern.edu [[6]mailto:histonet-bounces@lists.utsouthwestern.e=] On Behalf Of Lee & Peggy Wenk Sent: Thursday, March 10, 21 6:39 PM To: Amador, Amanda; [7]histonet@lists.utsouthwestern.edu Subject: Re: [Histone= Control Slides I've noticed that our spirochete control slides do=t stain as intense starting about 3 months after they are sectioned, =d that they stop staining by about 6 months. (This is with a silver s=in.) I've talked with other histotechs, and they say they've seen =e same phenomenon with AFB and Gram controls. (We use ours up too qui=ly - we never have 6-12 months old control slides for these, so I can= attest to this. ) Bancroft's book talks about this phenome=n with amyloid, and suggests that oxidation of the tissue (protein= due to exposure to air may be the cause. I'm guessing the it prob�ly applies to the precut microorganism control slides, too. We only cut enough slides for 6 months, and date them. Other people say they put their cut control slides in a slide box with a lid after dryi=, and then place the box in the refrig, as cold slows down the che=cal change, and the lid keeps the moving air off the slide. Other =ople say that, after they dry the slide, they dip the slide in par�fin, to cover the tissue, so that the air doesn't touch the tissue=ring the months before the slide is used in a stain. Just 3 sugge=ions to stop this problem. Peggy A. Wenk, HTL(ASCP)SLS Beaumont=spital Royal Oak, MI 48073 -----------------------------------=------------- From: "Amador, Amanda" <[8]aama...@ameripath.com> Sent: Wednesday, March 09, 2010:22 PM To: <[9]=sto...@lists.utsouthwestern.edu> Subject: [Histonet] Control Sl=es > Is there guidelines for how long special stains controls a= good for once > they are cut? We have spirochetes for our Stei=r that is from 2007 and > we are having issues. > >=anda Amador, HT(ASCP)CM > AmeriPath | Histology Group Lead/Trainer=560 N Shadeland Ave, Suite A | > Indianapolis, IN 46219 | phon�17.275.8052 | > [10]aamador@=eripath.com<mailto:[11]aam�o...@ameripath.com>; | > [12]www.AmeriPath.com<http://www.ameripath.c=/>; > > _______________________________________________ > Histonet mailing list > [13]Histonet@lists.utsouthwestern.edu > [14]http://lists.utso=hwestern.edu/mailman/listinfo/histonet __________________=___________________________ Histonet mailing list [15]Histonet@lists.utsouthwestern.edu[16]htt p://lists.utsouthwestern.edu/mailman/listinfo/histonet _________=____________________________________ Histonet mailing list [17]Histonet@lists.utsouthwestern=du [18]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. file://localhost/tmp/3D"ma 2. file://localhost/tmp/3D"mailto 3. 3D"mailto:aama...@ameripath.com"; 4. 3D"mailto:histonet@lists.utsouthwestern.edu"; 5. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu"; 6. 3D"mailto:histonet-bounc 7. 3D"mailto:histonet@lists.utsouth 8. 3D"mailto:aamador@ame 9. 3D"mailto:histonet@lists.utsouthwestern.edu"; 10. 3D"mailto:aama...@ameripath.com"; 11. 3D"http://aama...@ameripath.com>"/ 12. 3D"http://www.AmeriPath.com&l= 13. 3D"mailto:histo...@lists.uts 14. file://localhost/tmp/3D"h 15. file://localhost/tmp/3D"mailto 16. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; 17. ="mailto:Histonet@lists.utsouthwestern.edu"; 18. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/his_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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