Yes, it is good to use the metabisulfite rinses to remove any excess schiff 
reagent after the schiff step. But after the metabisulfite rinses, you still 
need to go into TAP water for several minutes to have the full color of the PAS 
reaction develop. It will get much brighter after the final tap water rinse.

amysue

______________________________________________________________________
The contents of this message may contain private, protected and/or privileged 
information.  If you received this message in error, you should destroy the 
e-mail message and any attachments or copies, and you are prohibited from 
retaining, distributing, disclosing or using any information contained within.  
Please contact the sender and advise of the erroneous delivery by return e-mail 
or telephone.  Thank you for your cooperation.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Reply via email to