Good morning! I'm looking for advice on an antibody conjugated with a blue fluorophore that could be used to tag for apoptosis (on p75 receptors on cholinergic neurons in the rat basal forebrain).
We use a Goat X ChAT primary (1:100) and a Rhodamine-conjugated Donkey X Goat secondary (1:250) on the slices. The slices already contain GFP expressed by the viral vector we infused into the basal forebrain. So, when we image the sections we already have a green fluorophore and a red fluorophore. Is there a blue fluorophore tag for apoptosis that anyone has used which would be biochemically compatible with the antibodies above? Is there another fluorescent option? Internet-searching and literature-reading has not gotten me anywhere. Our scope is a Leica DM4000B equipped with a Leica DFC 425C camera. Thanks for your time; any advice or insight would be much appreciated. Sincerely, Britt Tracy (undergraduate researcher at Temple University) _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
