I use the NovaCastra MSH-2 and MLH-1 on the Leica Bond Maxx. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, March 24, 2011 9:49 AM To: Hoekert, W.E.J.; Evans, Andria B; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Microsatellite Instability Antibodies We use Leica Bond Max and Biocare antibodies work very well for us. We're working on perfecting the Leica antibodies as well. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hoekert, W.E.J. Sent: Thursday, March 24, 2011 11:18 To: Evans, Andria B; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microsatellite Instability Antibodies >We are currently running the Ventana IHC stainers with the UltraView Detection. Just like us! MLH-1: Cell Marque (ref 760-4264). 30' cc1, 32' ab incubation. MSH-2: Cell Marque (ref 760-4265). 60' cc1, 60' ab inc. PMS2: Becton Dickinson, clone A16-4, cat nr 556415. 30' cc1, 32' ab inc. +amplification MSH6: Becton Dickinson, clone 44, cat nr 610919. 60' cc2, 60' ab inc. MSH-6 does not give very nice results, however, it works. Good Luck, Willem ________________________________ Van: histonet-boun...@lists.utsouthwestern.edu namens Evans, Andria B Verzonden: do 24-3-2011 13:31 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Microsatellite Instability Antibodies I am currently working up the MSI Antibodies (MLH1, MSH6, MSH2, and PMS2) and need some input on the protocol that you may be running in your lab and what clone(manufacture) you are using. We are currently running the Ventana IHC stainers with the UltraView Detection. Thanks for your help! Andria B Evans HTL(ASCP)CM This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet