I'm curious what Giemsa stain method people are using for frozen sections? I have a simple Wright-Giemsa stain that I use for blood smears (using a 1 min. methanol fix, 30 second stain with Wright-Giemsa, 5 minute in Phosphate buffer and then rinse in dH20). The investigator sent me a method using a HEPES buffer. It is involved to make up the working buffer of HEPES. I would appreciate any input.
Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
