Yes, that could be true. Some of the PrepKits "go harder" than others. Perhaps the pressure of the hammer is not big enough. Sometimes the nozzles are not filled equally. (We press always reagens in the nozzle when we prepare the run.)
With the Ventana-System there is an additional dilution, because the working-solution is added to a reaction-buffer film under the LCS. Perhaps it makes a difference if you dispense the solution with the pipett-tip under the LCS or if the drop falls on the surface of the LCS. That could be a matter of the LCS-quality, or buffer-quantity , or . I think I'll become mad! Our pathologists have an uncertain unhappiness with the overall staining results, but most of the antibodies work well. Perhaps the majority isn't very sensitiv to small changes in the system and the quality-difference isn't big enough, but some are easier to "kill". Regards, Gudrun _____ Von: Angela Bitting [mailto:akbitt...@geisinger.edu] Gesendet: Montag, 11. April 2011 18:06 An: gu.l...@gmx.at Betreff: Re: [Histonet] Benchmark Ultra question When I was trained to do titration runs on the BenchmarkXT and Ultras, I was told to titrate 100ul of antibody. I have been suspecting for some time now that the dispensers DON'T dispense a full 100ul. I haven't taken the time to prove it, but your question may have motivated me. That could explain the weak staining. >>> "Gudrun Lang" <gu.l...@gmx.at> 4/11/2011 11:48 AM >>> Hi! I have some issues with our new Ultra. Since a few days antibodies, that work usually well, show up very faint. For example the TTF1 (Novocastra). I ordered a new bottle, made a titration and found, that the "old" titer 1:50 was again well enough. I filled the old PrepKit - and the result was very weak. Then I thought, the PrepKit itself is the culprit. I changed the PrepKit - and again the result was very weak. At that time the working-solution was only three days old. Has anyone an explanation, why the titration works well and the automated dispension doesn't? Regards Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____ IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet