Are you letting the tape sit out ("cure") for awhile befoe you use it? We leave
ours out overnight?
Lisa
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bales, Candy A
Sent: Tuesday, April 12, 2011 8:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue-Tek cover slipper question
Good morning. I work part-time at a small lab and they have a Sakura Feintek
Tissue-Tek SCA tape coverslipper, model 4764.
I have not worked with any tape coverslippers before and am having problems
with areas of the tissue drying out. The solvent bottle is full of Xylene.
However, there is a crack in the lid of the bottle.
After removing the stained slides from the autostainer and loading the
basket(s) to place on the cover slipper, I re-dip the slides in Xylene.
The room where this equipment is housed, does get warm. I was wondering if the
tape can become old & brittle, leading to it not sealing well, or if not enough
Xylene is being deposited on each slide or a combination of both?
I could not find the manual on the unit for trouble shooting ideas.
Any help would be greatly appreciated.
Thank you
Candy
Candy Bales, HT
Chief Histologist
The University of Texas Dental Branch at Houston
Diagnostic Sciences-Oral Pathology
6516 M.D. Anderson Blvd. # 3.093
Houston, TX 77030
713.500.4411 office
713.500.4416 fax
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