You could do that or you could use DAB for one antibody ("brownish" color),
followed by DAB + Nickel for the other ("bluish" color) and they are both
permanent and alcohol resistant.
René J.
--- On Tue, 4/12/11, Eva Permaul <e...@georgetown.edu> wrote:
From: Eva Permaul <e...@georgetown.edu>
Subject: [Histonet] Counterstain for dual chromogenic
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, April 12, 2011, 11:37 AM
Good morning,
I have been attempting to do some dual chromogenic staining. I am using a mouse
and a rabbit antibody. For the mouse antibody I am using an HRP-mouse secondary
followed by AEC for visualization. For the rabbit antibody I am using a
biotinylated rabbit secondary, followed by ABC-AP and vector blue. My problem
is what to use as a counterstain. From everything I have read so far there
isn't one that would work without having some problems. I was thinking of
trying a very light Hematoxylin and see if it doesn't disrupt the vector blue
visualization too much. Does anyone else have any suggestions?
Thanks,
Eva
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