I purchase a kit from Dorn and Hart Microedge and use sodium carbonate-formaldehyde solution to chemically develop.
Stain in silver nitrate for 5 min in the dark, 3 fresh DI rinses for 1 min each in the dark, then sodium carbonate-formaldehyde solution for 2 min in the dark, 2 fresh DI rinses (normal light) for 1 min each, 30 seconds in Farmer's Diminisher (Sodium thiosulfate-potassium ferricyanide soluiton to stop reaction) and a running tap water rinse for 10 min. Jack > From: dorothy.l.w...@healthpartners.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 12 Apr 2011 12:35:18 -0500 > Subject: [Histonet] Von kossa stain > > What is everyone using for their "light" when developing the silver in the > VonKossa stain when you have no sunlight to use? We used to use a 60 watt > lamp, but haven't done one for years and am bringing this stain back to our > repetiore due to pathologist request. Thanks much! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Supervisor > 651-254-2962 > > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
