I would consider replacing the sponges. Tony is correct that you need to make sure the air is removed from the sponge to facilitate exchange, but with shortened processing times you will undoubtedly cause carry over from solution to solution. Yuo may want to have a small container of alcoholic formalin with sponges sitting on the gross table.
Try using a nylon tissue bag to funnel filter your small biopsies. all your biopsy sample sizes can be placed into the bag and very tiny samples are retained. Last suggestion is to consider a folded filter paper method (we name it origami, I can provide directly if you want) that uses a quick four fold process w/ forceps at the gross bench to create a pocket for the tiny samples, has a single layer between the tissue/solution and is easy to open at embedding (without "popping" of tissue samples). Avoind the unnecessary carryover of the sponge if you can. William DeSalvo, B.S., HTL(ASCP) > From: antho...@chw.edu.au > To: aeva...@lghealth.org; histonet@lists.utsouthwestern.edu > Date: Tue, 12 Apr 2011 23:30:30 +0000 > CC: > Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor > > Increase the fixation time and make sure the air is out of the sponges (will > stop formalin from getting to the tissue- a quick dunk of the cassette (with > sponge and tissue) in alcohol and back into formalin will do the trick). > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Evans, Andria > B > Sent: Wednesday, 13 April 2011 4:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor > > Our lab is currently looking for a way to shorten our Biopsy processing > program without compromising the patient specimen. We do have an issue with > our GI's being very dry, which causes us to have to soak between each level > taken and also causes a lot of chatter. Also we have a goal to do a run > during the day to improve turn around time. Here is what our current > protocol is.... > > Formalin 1 min 37degrees > Formalin 15 mins 37degrees > 70 14 mins 40 degrees > 95 14 mins 40 degrees > 95 9 mins 40 degrees > 100 9 mins 40 degrees > 100 7 mins 40 degrees > 100 4 mins 40 degrees > Xylene 23 mins no heat > Xylene 15 mins no heat > Paraffin 20 mins 60 degrees > Paraffin 18 mins 60 degrees > Paraffin 10 mins 60 degrees > Paraffin 0 mins 60 degrees > > All the steps are set on a fast mix setting. All of our biopsy specimens are > put into sponges. > > Any feedback would be greatly appreciated. > > Andria B Evans HTL(ASCP)CM > Lancaster General Hospital > 555 North Duke Street > Lancaster, PA 17604 > (717)544-5511 ext: 77329 > aeva...@lgheath.org<mailto:aeva...@lgheath.org> > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use of > intended recipient(s) and may contain confidential and privileged > information. > Any unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
