Mahesh- If you have access to a sonicator, you can etch the slides by first soaking them in 1% formic acid in the sonicator for 5-10 minutes, then after a brief water rinse soak them in 50% ethanol for another 5-10 minutes in the sonicator.
You may have to work with the staining times, but you will find that many of your paraffin embedding stains will work. Good luck! Joe Saby, BA HT NAMSA ________________________________ From: Mahesh Polavarapu <polavarapu.mah...@gmail.com> To: histonet <histonet@lists.utsouthwestern.edu> Sent: Tue, April 12, 2011 8:25:30 PM Subject: [Histonet] Update regarding question on Plastic Embedding Looking for a protocol to visualize vascularization and collagen deposition at the bone-tendon interface of a rabbit rotator cuff embedded in a plastic system. Sections will be rather thick (~50um) b/c they are being made through a titanium anchor. Using MMA with a cold-curing resin, Technovit 9100. Thanks in advance! - Mahesh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
