Hey ya'll, So I am trying to do IHC on paraffin embedded cell blocks. The cell blocks were made in an agar solution. The H&E slides came out fine and look like a normal cell block slide. However I am now a little confused. I put the slides through HIER and it doesn't look like there is anything on the slide anymore? Are the cells still there, just the agar fell off because of the heat? Should I continue to use all the reagents and antibody on these slides? Also, if they are trash...how do I do IHC stains on these blocks? I do not have access to a cytospin machine so my "traditional" way of doing ICC with cytospins I cannot do. Ahhh!! Help!!!
Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
