What about using it on specimens that are going to be tested for
prognostic markers? Like Her 2 neu? Thanks Deb.
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of
[email protected]
Sent: Wednesday, April 13, 2011 9:10 AM
To: [email protected]
Subject: Histonet Digest, Vol 89, Issue 13
Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Immunofluorescence and FISH Combined stating (Diane Kolins)
2. Von kossa stain (Webb, Dorothy L)
3. RE: Von kossa stain (Breeden, Sara)
4. Re: Histonet Digest, Vol 89, Issue 12 (Mark Elliott)
5. AW: [Histonet] Von kossa stain (Gudrun Lang)
6. Re: AW: [Histonet] Von kossa stain
(Grantham, Andrea L - (algranth))
7. Staining using a Plastic Sytem (Mahesh Polavarapu)
8. Biopsy program for Sakura VIP 5/6 Processor (Evans, Andria B)
9. Re: Milestone MW (Rene J Buesa)
10. Re: Von kossa stain (Rene J Buesa)
11. RE: Von kossa stain (Jack Ratliff)
12. RE: Counterstain for dual chromogenic (Tony Henwood)
13. RE: Von kossa stain (Tony Henwood)
14. RE: Biopsy program for Sakura VIP 5/6 Processor (Tony Henwood)
15. RE: RE: Biopsy program for Sakura VIP 5/6 Processor
(WILLIAM DESALVO)
16. RE: RE: Biopsy program for Sakura VIP 5/6 Processor (Tony
Henwood)
17. Update regarding question on Plastic Embedding (Mahesh
Polavarapu)
18. SP3 HER2 mAb (Richard Cartun)
19. FW: 1 H&E slide vs. 2 (Eugenia Thomas)
20. Ventana ultra ([email protected])
21. RE: FW: 1 H&E slide vs. 2 (Monfils, Paul)
22. RE: Ventana ultra (Sebree Linda A)
----------------------------------------------------------------------
Message: 1
Date: Tue, 12 Apr 2011 13:23:03 -0400
From: Diane Kolins <[email protected]>
Subject: [Histonet] Immunofluorescence and FISH Combined stating
To: [email protected]
Message-ID:
<!&!AAAAAAAAAAAYAAAAAAAAAKxbzBNp1TJAsnIO9XHEfgnCgAAAEAAAAKFMd/[email protected]>
Content-Type: text/plain; charset=us-ascii
Where can I find a procedure for dual staining of blood and bone
marrow
smears using Vector anti-kappa and anti-lambda tagged with Coumarine
followed by FISH hybridization. I am able to see what I think are
plasma
cells by their fluorescent blue cytoplasm and on the same slide
(changing
filters) I can see the red and green signal patterns.
From: : [email protected]
------------------------------
Message: 2
Date: Tue, 12 Apr 2011 12:35:18 -0500
From: "Webb, Dorothy L" <[email protected]>
Subject: [Histonet] Von kossa stain
To: "'[email protected]'"
<[email protected]>
Message-ID:
<65365f35c0f2ef4d846ec3ca73e49c43010f85105...@hpemx3.healthpartners.int>
Content-Type: text/plain; charset="us-ascii"
What is everyone using for their "light" when developing the silver in
the VonKossa stain when you have no sunlight to use? We used to use a
60 watt lamp, but haven't done one for years and am bringing this
stain back to our repetiore due to pathologist request. Thanks much!
Dorothy Webb, HT (ASCP)
Regions Histology Technical Supervisor
651-254-2962
________________________________
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient,
please be advised that you have received this e-mail in error and that
any use, dissemination, forwarding, printing, or copying of this
e-mail is strictly prohibited.
If you have received this e-mail in error, please immediately notify
the HealthPartners Support Center by telephone at (952) 967-6600. You
will be reimbursed for reasonable costs incurred in notifying us.
HealthPartners R001.0
------------------------------
Message: 3
Date: Tue, 12 Apr 2011 11:52:49 -0600
From: "Breeden, Sara" <[email protected]>
Subject: RE: [Histonet] Von kossa stain
To: "Webb, Dorothy L" <[email protected]>,
<[email protected]>
Message-ID:
<4d14f0fc9316dd41972d5f03c070908b02e47...@nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
Funny you should ask... just last week I had two requests for a Von
Kossa. That's when I found out that, despite the fact that we just
moved into a brand new building with all the bells and whistles, there
was not one single UV light in any of the many hoods that had been
installed. Heaven forbid I could even find an incandescent bulb (we
are a "green building"). So, I prepped my slides, put them in a clear
glass Coplin jar and parked the jar on the hood of my car for an hour.
Works like a charm. This all depends, naturally, on (1) if you even
HAVE sunshine where you live; (2) how far a walk it is to the
sunshine,
and (3) whether you have a car hood on which to park the Coplin jar.
Needless to say, out of the line of sight of curious onlookers with
sticky fingers and no business wondering what that glass jar is...
Nonetheless, it works just fine! May the Force be with you.
------------------------------
Message: 4
Date: Tue, 12 Apr 2011 11:16:16 -0700
From: "Mark Elliott" <[email protected]>
Subject: [Histonet] Re: Histonet Digest, Vol 89, Issue 12
To: <[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset=US-ASCII
Date: Tue, 12 Apr 2011 11:37:17 -0400
From: Eva Permaul <[email protected]>
Subject: [Histonet] Counterstain for dual chromogenic
To: [email protected]
Message-ID: <[email protected]>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Eva
I have been doing some double labelling using Vulcan Fast Red from
Biocare and Vector Blue Alkaline Phosphatase Substrate Kit III from
Vector Labs. For counter stain I use Vector Methyl Green and the
combination works great and looks quite nice.
Mark
Good morning,
I have been attempting to do some dual chromogenic staining. I am
using a mouse and a rabbit antibody. For the mouse antibody I am using
an HRP-mouse secondary followed by AEC for visualization. For the
rabbit antibody I am using a biotinylated rabbit secondary, followed
by ABC-AP and vector blue. My problem is what to use as a
counterstain. From everything I have read so far there isn't one that
would work without having some problems. I was thinking of trying a
very light Hematoxylin and see if it doesn't disrupt the vector blue
visualization too much. Does anyone else have any suggestions?
Thanks,
Eva
***CONFIDENTIALITY NOTICE***
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------------------------------
Message: 5
Date: Tue, 12 Apr 2011 20:22:22 +0200
From: "Gudrun Lang" <[email protected]>
Subject: AW: [Histonet] Von kossa stain
To: "'Webb, Dorothy L'" <[email protected]>
Cc: [email protected]
Message-ID: <[email protected]>
Content-Type: text/plain; charset="iso-8859-1"
WE use just a simple desk-lamp and put the coplin jar directly under
the
bulb. And the desk-lamp is placed in the window.
Bye Gudrun
-----Ursprüngliche Nachricht-----
Von: [email protected]
[mailto:[email protected]] Im Auftrag von
Webb,
Dorothy L
Gesendet: Dienstag, 12. April 2011 19:35
An: '[email protected]'
Betreff: [Histonet] Von kossa stain
What is everyone using for their "light" when developing the silver in
the
VonKossa stain when you have no sunlight to use? We used to use a 60
watt
lamp, but haven't done one for years and am bringing this stain back
to our
repetiore due to pathologist request. Thanks much!
Dorothy Webb, HT (ASCP)
Regions Histology Technical Supervisor
651-254-2962
________________________________
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they
are
addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient,
please be
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If you have received this e-mail in error, please immediately notify
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------------------------------
Message: 6
Date: Tue, 12 Apr 2011 11:35:47 -0700
From: "Grantham, Andrea L - (algranth)" <[email protected]>
Subject: Re: AW: [Histonet] Von kossa stain
Cc: HISTONET <[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset="iso-8859-1"
We have intense sun here but my windows face the wrong way! At least I
have windows. I just use a light bulb from the lamp we have over the
coverslipping station. Works great.
Andi
On Apr 12, 2011, at 11:22 AM, Gudrun Lang wrote:
WE use just a simple desk-lamp and put the coplin jar directly under
the
bulb. And the desk-lamp is placed in the window.
Bye Gudrun
-----Ursprüngliche Nachricht-----
Von: [email protected]
[mailto:[email protected]] Im Auftrag von
Webb,
Dorothy L
Gesendet: Dienstag, 12. April 2011 19:35
An: '[email protected]'
Betreff: [Histonet] Von kossa stain
What is everyone using for their "light" when developing the silver
in the
VonKossa stain when you have no sunlight to use? We used to use a 60
watt
lamp, but haven't done one for years and am bringing this stain back
to our
repetiore due to pathologist request. Thanks much!
Dorothy Webb, HT (ASCP)
Regions Histology Technical Supervisor
651-254-2962
________________________________
This e-mail and any files transmitted with it are confidential and
are
intended solely for the use of the individual or entity to whom they
are
addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient,
please be
advised that you have received this e-mail in error and that any use,
dissemination, forwarding, printing, or copying of this e-mail is
strictly
prohibited.
If you have received this e-mail in error, please immediately notify
the
HealthPartners Support Center by telephone at (952) 967-6600. You
will be
reimbursed for reasonable costs incurred in notifying us.
HealthPartners
R001.0
_______________________________________________
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_______________________________________________
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------------------------------
Message: 7
Date: Tue, 12 Apr 2011 13:56:53 -0500
From: Mahesh Polavarapu <[email protected]>
Subject: [Histonet] Staining using a Plastic Sytem
To: [email protected]
Message-ID: <[email protected]>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
Does anyone have experience embedding and staining in plastic? We have
rabbit rotator cuff tendons that are being embedded in plastic. I am
trying
to figure out what the process is and which stains to use in order to
visualize (and quantify) blood vessels and collagen deposition at the
Bone
(Humeral Head) - Tendon (Infraspinatus) interface.
Thanks,
Mahesh
------------------------------
Message: 8
Date: Tue, 12 Apr 2011 14:57:18 -0400
From: "Evans, Andria B" <[email protected]>
Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
To: "[email protected]"
<[email protected]>
Message-ID:
<4182fdf23d7c9948bc41c4c082c3a54f021557b3a...@mail-ag-cluster.lha.org>
Content-Type: text/plain; charset="iso-8859-1"
Our lab is currently looking for a way to shorten our Biopsy
processing program without compromising the patient specimen. We do
have an issue with our GI's being very dry, which causes us to have to
soak between each level taken and also causes a lot of chatter. Also
we have a goal to do a run during the day to improve turn around time.
Here is what our current protocol is....
Formalin 1 min 37degrees
Formalin 15 mins 37degrees
70 14 mins 40 degrees
95 14 mins 40 degrees
95 9 mins 40 degrees
100 9 mins 40 degrees
100 7 mins 40 degrees
100 4 mins 40 degrees
Xylene 23 mins no heat
Xylene 15 mins no heat
Paraffin 20 mins 60 degrees
Paraffin 18 mins 60 degrees
Paraffin 10 mins 60 degrees
Paraffin 0 mins 60 degrees
All the steps are set on a fast mix setting. All of our biopsy
specimens are put into sponges.
Any feedback would be greatly appreciated.
Andria B Evans HTL(ASCP)CM
Lancaster General Hospital
555 North Duke Street
Lancaster, PA 17604
(717)544-5511 ext: 77329
[email protected]<mailto:[email protected]>
This email was sent securely from the LGHealth Email Service
Confidentiality Notice: This e-mail message, including any
attachments, is for the sole use
of intended recipient(s) and may contain confidential and
privileged information. Any unauthorized review, use, disclosure or
distribution is
prohibited. If you are not the intended recipient, please contact the
sender by
reply e-mail and destroy all copies of the original message.
------------------------------
Message: 9
Date: Tue, 12 Apr 2011 12:27:49 -0700 (PDT)
From: Rene J Buesa <[email protected]>
Subject: Re: [Histonet] Milestone MW
To: [email protected], SHANE NELSON
<[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset=iso-8859-1
Model "KOS" by Milestone is capable of tissue processing,
decalcification, special stains, fixation, gross hardening, and
antigen retrieval.
René J.
--- On Tue, 4/12/11, SHANE NELSON <[email protected]> wrote:
From: SHANE NELSON <[email protected]>
Subject: [Histonet] Milestone MW
To: [email protected]
Date: Tuesday, April 12, 2011, 12:30 PM
Margret,
Which microwave would that be
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP) LABORATORY CONSULTANT
SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS
35-900 Bob Hope Drive
Suite 275
Rancho Mirage, Ca. 92270
cell (909) 841-9761 / wk (760) 321-2500
[email protected]
_______________________________________________
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------------------------------
Message: 10
Date: Tue, 12 Apr 2011 12:30:26 -0700 (PDT)
From: Rene J Buesa <[email protected]>
Subject: Re: [Histonet] Von kossa stain
To: "'[email protected]'"
<[email protected]>, Dorothy LWebb
<[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset=iso-8859-1
You can use any strong intensity microscope light. The stronger
the intensity of the light the better, but the reduction has a
cumulative effect so even not very strong intensity bulbs can be
effective.
René J.
--- On Tue, 4/12/11, Webb, Dorothy L
<[email protected]> wrote:
From: Webb, Dorothy L <[email protected]>
Subject: [Histonet] Von kossa stain
To: "'[email protected]'"
<[email protected]>
Date: Tuesday, April 12, 2011, 1:35 PM
What is everyone using for their "light" when developing the silver in
the VonKossa stain when you have no sunlight to use? We used to use a
60 watt lamp, but haven't done one for years and am bringing this
stain back to our repetiore due to pathologist request. Thanks much!
Dorothy Webb, HT (ASCP)
Regions Histology Technical Supervisor
651-254-2962
________________________________
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient,
please be advised that you have received this e-mail in error and that
any use, dissemination, forwarding, printing, or copying of this
e-mail is strictly prohibited.
If you have received this e-mail in error, please immediately notify
the HealthPartners Support Center by telephone at (952) 967-6600. You
will be reimbursed for reasonable costs incurred in notifying us.
HealthPartners R001.0
_______________________________________________
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------------------------------
Message: 11
Date: Tue, 12 Apr 2011 15:41:54 -0400
From: Jack Ratliff <[email protected]>
Subject: RE: [Histonet] Von kossa stain
To: <[email protected]>, Histonet
<[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset="iso-8859-1"
I purchase a kit from Dorn and Hart Microedge and use sodium
carbonate-formaldehyde solution to chemically develop.
Stain in silver nitrate for 5 min in the dark, 3 fresh DI rinses for 1
min each in the dark, then sodium carbonate-formaldehyde solution for
2 min in the dark, 2 fresh DI rinses (normal light) for 1 min each, 30
seconds in Farmer's Diminisher (Sodium thiosulfate-potassium
ferricyanide soluiton to stop reaction) and a running tap water rinse
for 10 min.
Jack
From: [email protected]
To: [email protected]
Date: Tue, 12 Apr 2011 12:35:18 -0500
Subject: [Histonet] Von kossa stain
What is everyone using for their "light" when developing the silver
in the VonKossa stain when you have no sunlight to use? We used to
use a 60 watt lamp, but haven't done one for years and am bringing
this stain back to our repetiore due to pathologist request. Thanks
much!
Dorothy Webb, HT (ASCP)
Regions Histology Technical Supervisor
651-254-2962
________________________________
This e-mail and any files transmitted with it are confidential and
are intended solely for the use of the individual or entity to whom
they are addressed. If you are not the intended recipient or the
individual responsible for delivering the e-mail to the intended
recipient, please be advised that you have received this e-mail in
error and that any use, dissemination, forwarding, printing, or
copying of this e-mail is strictly prohibited.
If you have received this e-mail in error, please immediately notify
the HealthPartners Support Center by telephone at (952) 967-6600. You
will be reimbursed for reasonable costs incurred in notifying us.
HealthPartners R001.0
_______________________________________________
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------------------------------
Message: 12
Date: Tue, 12 Apr 2011 23:11:35 +0000
From: Tony Henwood <[email protected]>
Subject: RE: [Histonet] Counterstain for dual chromogenic
To: "'Eva Permaul'" <[email protected]>,
"[email protected]"
<[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset="us-ascii"
Try ethyl green:
1. 0.1N Acetic Acid
Add 6ml of glacial acetic acid to 1000 ml of distilled water. 2.
0.1N Sodium Acetate
Add 4.102 g of sodium acetate to 500 ml of distilled water
3. pH 4.2 buffer
Add 755 ml of 0.1N acetic acid to 264 ml of 0.1N Sodium Acetate and
adjust the pH to 4.2 with 1M NaOH
4. Ethyl Green Solution
To 100ml of buffer add 2g Ethyl Green (CI 42590)
Can be stored at room temp. If staining turns bluish than the
solution has started to deteriorate.
Stain for 5 minutes. Do not rinse in water for too long, it tends to
extract the green staining
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),
FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306
Fax: 612 9845 3318 the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of Eva
Permaul
Sent: Wednesday, 13 April 2011 1:37 AM
To: [email protected]
Subject: [Histonet] Counterstain for dual chromogenic
Good morning,
I have been attempting to do some dual chromogenic staining. I am
using a mouse and a rabbit antibody. For the mouse antibody I am using
an HRP-mouse secondary followed by AEC for visualization. For the
rabbit antibody I am using a biotinylated rabbit secondary, followed
by ABC-AP and vector blue. My problem is what to use as a
counterstain. From everything I have read so far there isn't one that
would work without having some problems. I was thinking of trying a
very light Hematoxylin and see if it doesn't disrupt the vector blue
visualization too much. Does anyone else have any suggestions?
Thanks,
Eva
_______________________________________________
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[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
*********************************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please delete it
and notify the sender.
Views expressed in this message and any attachments are those of the
individual sender, and are not necessarily the views of The Children's
Hospital at Westmead
This note also confirms that this email message has been virus scanned
and although no computer viruses were detected, The Childrens Hospital
at Westmead accepts no liability for any consequential damage
resulting from email containing computer viruses.
*********************************************************************************
------------------------------
Message: 13
Date: Tue, 12 Apr 2011 23:22:21 +0000
From: Tony Henwood <[email protected]>
Subject: RE: [Histonet] Von kossa stain
To: "'Breeden, Sara'" <[email protected]>, "Webb, Dorothy L"
<[email protected]>,
"[email protected]"
<[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset="us-ascii"
Interesting,
Meloan & Puchtler (1985) have drawn our attention to Von Kossa's
original work on this technique. He regarded only the yellow
colouration of calcium deposits during early stages of the reaction as
diagnostic for calcium phosphate and credited the blackening to
organic matter. Further studies showed that bright light only causes
the irreversible blackening of organic matter that masks the yellow
silver phosphate. When the reaction is performed in subdued light,
yellow to yellowish brown silver phosphate is visualised selectively.
Silver carbonate dissolves in sodium thiosulphate and cannot be
demonstrated with von Kossa's technique (Meloan & Puchtler 1985 J
Histotechnol 8(1):11-13.).
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),
FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306
Fax: 612 9845 3318 the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of
Breeden, Sara
Sent: Wednesday, 13 April 2011 3:53 AM
To: Webb, Dorothy L; [email protected]
Subject: RE: [Histonet] Von kossa stain
Funny you should ask... just last week I had two requests for a Von
Kossa. That's when I found out that, despite the fact that we just
moved into a brand new building with all the bells and whistles, there
was not one single UV light in any of the many hoods that had been
installed. Heaven forbid I could even find an incandescent bulb (we
are a "green building"). So, I prepped my slides, put them in a clear
glass Coplin jar and parked the jar on the hood of my car for an hour.
Works like a charm. This all depends, naturally, on (1) if you even
HAVE sunshine where you live; (2) how far a walk it is to the
sunshine, and (3) whether you have a car hood on which to park the
Coplin jar.
Needless to say, out of the line of sight of curious onlookers with
sticky fingers and no business wondering what that glass jar is...
Nonetheless, it works just fine! May the Force be with you.
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
*********************************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please delete it
and notify the sender.
Views expressed in this message and any attachments are those of the
individual sender, and are not necessarily the views of The Children's
Hospital at Westmead
This note also confirms that this email message has been virus scanned
and although no computer viruses were detected, The Childrens Hospital
at Westmead accepts no liability for any consequential damage
resulting from email containing computer viruses.
*********************************************************************************
------------------------------
Message: 14
Date: Tue, 12 Apr 2011 23:30:30 +0000
From: Tony Henwood <[email protected]>
Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor
To: "'Evans, Andria B'" <[email protected]>,
"[email protected]"
<[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset="us-ascii"
Increase the fixation time and make sure the air is out of the sponges
(will stop formalin from getting to the tissue- a quick dunk of the
cassette (with sponge and tissue) in alcohol and back into formalin
will do the trick).
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),
FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306
Fax: 612 9845 3318 the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of Evans,
Andria B
Sent: Wednesday, 13 April 2011 4:57 AM
To: [email protected]
Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
Our lab is currently looking for a way to shorten our Biopsy
processing program without compromising the patient specimen. We do
have an issue with our GI's being very dry, which causes us to have to
soak between each level taken and also causes a lot of chatter. Also
we have a goal to do a run during the day to improve turn around time.
Here is what our current protocol is....
Formalin 1 min 37degrees
Formalin 15 mins 37degrees
70 14 mins 40 degrees
95 14 mins 40 degrees
95 9 mins 40 degrees
100 9 mins 40 degrees
100 7 mins 40 degrees
100 4 mins 40 degrees
Xylene 23 mins no heat
Xylene 15 mins no heat
Paraffin 20 mins 60 degrees
Paraffin 18 mins 60 degrees
Paraffin 10 mins 60 degrees
Paraffin 0 mins 60 degrees
All the steps are set on a fast mix setting. All of our biopsy
specimens are put into sponges.
Any feedback would be greatly appreciated.
Andria B Evans HTL(ASCP)CM
Lancaster General Hospital
555 North Duke Street
Lancaster, PA 17604
(717)544-5511 ext: 77329
[email protected]<mailto:[email protected]>
This email was sent securely from the LGHealth Email Service
Confidentiality Notice: This e-mail message, including any
attachments, is for the sole use of intended recipient(s) and may
contain confidential and privileged information. Any unauthorized
review, use, disclosure or distribution is prohibited. If you are not
the intended recipient, please contact the sender by reply e-mail and
destroy all copies of the original message.
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Message: 15
Date: Tue, 12 Apr 2011 17:58:36 -0600
From: WILLIAM DESALVO <[email protected]>
Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6
Processor
To: <[email protected]>, <[email protected]>, histonet
<[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset="iso-8859-1"
I would consider replacing the sponges. Tony is correct that you need
to make sure the air is removed from the sponge to facilitate
exchange, but with shortened processing times you will undoubtedly
cause carry over from solution to solution. Yuo may want to have a
small container of alcoholic formalin with sponges sitting on the
gross table.
Try using a nylon tissue bag to funnel filter your small biopsies. all
your biopsy sample sizes can be placed into the bag and very tiny
samples are retained. Last suggestion is to consider a folded filter
paper method (we name it origami, I can provide directly if you want)
that uses a quick four fold process w/ forceps at the gross bench to
create a pocket for the tiny samples, has a single layer between the
tissue/solution and is easy to open at embedding (without "popping" of
tissue samples). Avoind the unnecessary carryover of the sponge if you
can.
William DeSalvo, B.S., HTL(ASCP)
From: [email protected]
To: [email protected]; [email protected]
Date: Tue, 12 Apr 2011 23:30:30 +0000
CC: Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6
Processor
Increase the fixation time and make sure the air is out of the
sponges (will stop formalin from getting to the tissue- a quick dunk
of the cassette (with sponge and tissue) in alcohol and back into
formalin will do the trick).
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC),
FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306
Fax: 612 9845 3318 the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of
Evans, Andria B
Sent: Wednesday, 13 April 2011 4:57 AM
To: [email protected]
Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
Our lab is currently looking for a way to shorten our Biopsy
processing program without compromising the patient specimen. We do
have an issue with our GI's being very dry, which causes us to have
to soak between each level taken and also causes a lot of chatter.
Also we have a goal to do a run during the day to improve turn around
time. Here is what our current protocol is....
Formalin 1 min 37degrees
Formalin 15 mins 37degrees
70 14 mins 40 degrees
95 14 mins 40 degrees
95 9 mins 40 degrees
100 9 mins 40 degrees
100 7 mins 40 degrees
100 4 mins 40 degrees
Xylene 23 mins no heat
Xylene 15 mins no heat
Paraffin 20 mins 60 degrees
Paraffin 18 mins 60 degrees
Paraffin 10 mins 60 degrees
Paraffin 0 mins 60 degrees
All the steps are set on a fast mix setting. All of our biopsy
specimens are put into sponges.
Any feedback would be greatly appreciated.
Andria B Evans HTL(ASCP)CM
Lancaster General Hospital
555 North Duke Street
Lancaster, PA 17604
(717)544-5511 ext: 77329
[email protected]<mailto:[email protected]>
This email was sent securely from the LGHealth Email Service
Confidentiality Notice: This e-mail message, including any
attachments, is for the sole use of intended recipient(s) and may
contain confidential and privileged information. Any unauthorized
review, use, disclosure or distribution is prohibited. If you are
not the intended recipient, please contact the sender by reply e-mail
and destroy all copies of the original message.
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*********************************************************************************
This email and any files transmitted with it are confidential and
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are addressed. If you are not the intended recipient, please delete
it and notify the sender.
Views expressed in this message and any attachments are those of the
individual sender, and are not necessarily the views of The
Children's Hospital at Westmead
This note also confirms that this email message has been virus
scanned and although no computer viruses were detected, The Childrens
Hospital at Westmead accepts no liability for any consequential
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------------------------------
Message: 16
Date: Wed, 13 Apr 2011 00:10:04 +0000
From: Tony Henwood <[email protected]>
Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6
Processor
To: "'WILLIAM DESALVO'" <[email protected]>,
"[email protected]" <[email protected]>, histonet
<[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset="us-ascii"
I agree
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
From: WILLIAM DESALVO [mailto:[email protected]]
Sent: Wednesday, 13 April 2011 9:59 AM
To: Tony Henwood; [email protected]; histonet
Subject: RE: [Histonet] RE: Biopsy program for Sakura VIP 5/6
Processor
I would consider replacing the sponges. Tony is correct that you need
to make sure the air is removed from the sponge to facilitate
exchange, but with shortened processing times you will undoubtedly
cause carry over from solution to solution. Yuo may want to have a
small container of alcoholic formalin with sponges sitting on the
gross table.
Try using a nylon tissue bag to funnel filter your small biopsies. all
your biopsy sample sizes can be placed into the bag and very tiny
samples are retained. Last suggestion is to consider a folded filter
paper method (we name it origami, I can provide directly if you want)
that uses a quick four fold process w/ forceps at the gross bench to
create a pocket for the tiny samples, has a single layer between the
tissue/solution and is easy to open at embedding (without "popping" of
tissue samples). Avoind the unnecessary carryover of the sponge if you
can.
William DeSalvo, B.S., HTL(ASCP)
From: [email protected]<mailto:[email protected]>
To: [email protected]<mailto:[email protected]>;
[email protected]<mailto:[email protected]>
Date: Tue, 12 Apr 2011 23:30:30 +0000
CC:
Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor
Increase the fixation time and make sure the air is out of the
sponges (will stop formalin from getting to the tissue- a quick dunk
of the cassette (with sponge and tissue) in alcohol and back into
formalin will do the trick).
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From:
[email protected]<mailto:[email protected]>
[mailto:[email protected]]<mailto:[mailto:[email protected]]>
On Behalf Of Evans, Andria B
Sent: Wednesday, 13 April 2011 4:57 AM
To:
[email protected]<mailto:[email protected]>
Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
Our lab is currently looking for a way to shorten our Biopsy
processing program without compromising the patient specimen. We do
have an issue with our GI's being very dry, which causes us to have
to soak between each level taken and also causes a lot of chatter.
Also we have a goal to do a run during the day to improve turn around
time. Here is what our current protocol is....
Formalin 1 min 37degrees
Formalin 15 mins 37degrees
70 14 mins 40 degrees
95 14 mins 40 degrees
95 9 mins 40 degrees
100 9 mins 40 degrees
100 7 mins 40 degrees
100 4 mins 40 degrees
Xylene 23 mins no heat
Xylene 15 mins no heat
Paraffin 20 mins 60 degrees
Paraffin 18 mins 60 degrees
Paraffin 10 mins 60 degrees
Paraffin 0 mins 60 degrees
All the steps are set on a fast mix setting. All of our biopsy
specimens are put into sponges.
Any feedback would be greatly appreciated.
Andria B Evans HTL(ASCP)CM
Lancaster General Hospital
555 North Duke Street
Lancaster, PA 17604
(717)544-5511 ext: 77329
[email protected]<mailto:[email protected]<mailto:[email protected]%3cmailto:[email protected]>>
This email was sent securely from the LGHealth Email Service
Confidentiality Notice:
This e-mail message, including any attachments, is for the sole use
of intended recipient(s) and may contain confidential and privileged
information.
Any unauthorized review, use, disclosure or distribution is
prohibited.
If you are not the intended recipient, please contact the sender by
reply e-mail and destroy all copies of the original message.
_______________________________________________
Histonet mailing list
[email protected]<mailto:[email protected]>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
*********************************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please delete
it and notify the sender.
Views expressed in this message and any attachments are those of the
individual sender, and are not necessarily the views of The
Children's Hospital at Westmead
This note also confirms that this email message has been virus
scanned and although no computer viruses were detected, The Childrens
Hospital at Westmead accepts no liability for any consequential
damage resulting from email containing computer viruses.
*********************************************************************************
_______________________________________________
Histonet mailing list
[email protected]<mailto:[email protected]>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Tue, 12 Apr 2011 19:25:30 -0500
From: Mahesh Polavarapu <[email protected]>
Subject: [Histonet] Update regarding question on Plastic Embedding
To: histonet <[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset=ISO-8859-1
Looking for a protocol to visualize vascularization and collagen
deposition
at the bone-tendon interface of a rabbit rotator cuff embedded in a
plastic
system. Sections will be rather thick (~50um) b/c they are being made
through a titanium anchor. Using MMA with a cold-curing resin,
Technovit
9100. Thanks in advance!
- Mahesh
------------------------------
Message: 18
Date: Tue, 12 Apr 2011 20:34:51 -0400
From: "Richard Cartun" <[email protected]>
Subject: [Histonet] SP3 HER2 mAb
To: "Histonet" <[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset=US-ASCII
Anyone using the HER2 rabbit monoclonal antibody (clone SP3) ever see
reactivity with skeletal muscle? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
------------------------------
Message: 19
Date: Tue, 12 Apr 2011 23:14:30 -0400
From: Eugenia Thomas <[email protected]>
Subject: [Histonet] FW: 1 H&E slide vs. 2
To: <[email protected]>
Message-ID: <[email protected]>
Content-Type: text/plain; charset="iso-8859-1"
From: [email protected]
To: [email protected]
Subject: 1 H&E slide vs. 2
Date: Mon, 11 Apr 2011 13:06:59 -0400
Good afternoon everyone,
Does anyone know of an article or statistics discussing the impact
(medical diagnosing) of cutting 2 H&E slides per block verses 1 for
all routine work?
Genia
------------------------------
Message: 20
Date: Wed, 13 Apr 2011 08:39:22 -0500
From: <[email protected]>
Subject: [Histonet] Ventana ultra
To: <[email protected]>
Message-ID:
<7da79ebdbd92bf408ef392413737878d3936b06...@nadcwpmsgcms01.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
Does anyone use the Benchmark Ultra - care to share your comments?
We are considering switching from the Benchmark XT to the Ultra but
would like to hear from users about this instrument.
Is there an increase in reagent cost?
Can you really add more slides without adding time to the run?
ANTOINETTE CRILL, MBA,CT(ASCP)
TEAM LEADER ANATOMIC PATHOLOGY
DANVILLE REGIONAL MEDICAL CENTER
(O) 434.799.4470
(F) 434.773.6806
E-mail: [email protected]<mailto:[email protected]>
------------------------------
Message: 21
Date: Wed, 13 Apr 2011 10:00:09 -0400
From: "Monfils, Paul" <[email protected]>
Subject: RE: [Histonet] FW: 1 H&E slide vs. 2
To: <[email protected]>
Message-ID:
<4ebff65383b74d49995298c4976d1d5e08020...@lsriexch1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="us-ascii"
When I was in clinical histology (I'm in research now) the
pathologists
didn't even want us putting two sections on a slide, because even
though
they knew a second serial section wasn't going to show anything the
first section didn't show, they felt ethically/legally bound to
examine
both sections if they were there, so they were investing twice the
time
and effort for the same return.
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of
Eugenia
Thomas
Sent: Tuesday, April 12, 2011 11:15 PM
To: [email protected]
Subject: [Histonet] FW: 1 H&E slide vs. 2
From: [email protected]
To: [email protected]
Subject: 1 H&E slide vs. 2
Date: Mon, 11 Apr 2011 13:06:59 -0400
Good afternoon everyone,
Does anyone know of an article or statistics discussing the impact
(medical diagnosing) of cutting 2 H&E slides per block verses 1 for
all
routine work?
Genia
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Wed, 13 Apr 2011 09:09:22 -0500
From: "Sebree Linda A" <[email protected]>
Subject: RE: [Histonet] Ventana ultra
To: <[email protected]>, <[email protected]>
Message-ID:
<f0b9fd4b28c80e43996ffd4408b198ae074...@uwhc-mail3.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Please "Reply All" as I'd like to hear feedback also.
Thanks.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of
[email protected]
Sent: Wednesday, April 13, 2011 8:39 AM
To: [email protected]
Subject: [Histonet] Ventana ultra
Does anyone use the Benchmark Ultra - care to share your comments?
We are considering switching from the Benchmark XT to the Ultra but
would like to hear from users about this instrument.
Is there an increase in reagent cost?
Can you really add more slides without adding time to the run?
ANTOINETTE CRILL, MBA,CT(ASCP)
TEAM LEADER ANATOMIC PATHOLOGY
DANVILLE REGIONAL MEDICAL CENTER
(O) 434.799.4470
(F) 434.773.6806
E-mail: [email protected]<mailto:[email protected]>
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 89, Issue 13
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