You will have to decalcify the bone first. Being so small pieces, I advise you to use EDTA and proceed with the regular tissue processing after the bone is decalcified. René J.
--- On Sat, 4/16/11, [email protected] <[email protected]> wrote: From: [email protected] <[email protected]> Subject: [Histonet] Sectioning undecalcified bone To: [email protected] Date: Saturday, April 16, 2011, 8:29 PM I have a sample that is 3 needle bone biopsies that I need to get an H&E and subsequently several 12um thick sections for further assays. The bone appears to not be decaled at all. I don't normally have bone to cut so I'm looking for suggestions. Aprill _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
